RBS

Part:BBa_B0037

Designed by: Barry Canton   Group: iGEM04_MIT   (2005-12-06)

Specialized RBS

RBS specific to dedicated ribosomes. This RBS is a modified version of B0036. The spacing between the SD sequence and the start codon has been increased by 5 bases to give the same spacing as used by Brink et al. The 5 bases are the same as those used by Brink. That means they form the first five bases of an XbaI site and no negative affects of using these bases are known at present.

This RBS did not work well and is no longer in use.

Usage and Biology

The bases I added downstream of the RBS sequence form a hairpin with the mixed connective site upstream of the RBS. The hairpin, has 7 pairing bases. The RBS is in the loop of the hairpin but given the stable secondary structure, this RBS was unlikely to ever work. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//rbs/prokaryote/constitutive/miscellaneous
//ribosome/prokaryote/custom
//chassis/prokaryote/ecoli
//direction/forward
//regulation/constitutive
Parameters
None