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Plasmid_Backbone
pSB1A3

Part:pSB1A3:Sequence Analysis

Designed by: Reshma Shetty & Tom Knight   Group: Antiquity   (2004-05-04)
Sequence analyses related to pSB1A3 (length: 2155bp)
pSB1A3
pSB1A3
The sequence analysis tools use a blast database that was last updated on Thu Feb 8 10:32:16 2024. (Update now)

Quickly compare one sequence to pSB1A3
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Advanced sequence analyses for pSB1A3 Login to start an advanced analysis
InconsistentPBB - pSB1A3vinoo2011-04-14
Inconsistent--BioBrick Blast 4743--marcmtk2009-10-21
Sequencing performed on BBa_K237002 in what should have been psB1A3 (Originally with the RFP generator BBa_J04450) extracted from Well 1K of Spring 2009 distribution plate 1. The sequencing shows that the backbone is in fact psB1A2
InconsistentQC08 Plate 632, Well 40225meagan2008-05-16
Sample is in Well: Spring 2008 Distribution->Source Plate 1008: 1C

Sequence and Features for pSB1A3
Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2134
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 2140
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2134
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 2134
    Illegal suffix found at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 2134
    Plasmid lacks a suffix.
    Illegal XbaI site found at 2149
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI.rc site found at 1173