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Composite

Part:BBa_K510021:Sequence Analysis

Designed by: David Caballero & Fernando Govantes   Group: iGEM11_UPO-Sevilla   (2011-09-19)
Sequence analyses related to BBa_K510021 (length: 12194bp)

K510012
K325909
The sequence analysis tools use a blast database that was last updated on Thu Feb 8 10:32:16 2024. (Update now)

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Sequence and Features for BBa_K510021
Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 4528
    Illegal suffix found in sequence at 1
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 4528
    Illegal NheI site found at 5762
    Illegal NheI site found at 8728
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 4534
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 4528
    Illegal BglII site found at 3212
    Illegal BglII site found at 3483
    Illegal BglII site found at 3769
    Illegal BglII site found at 7726
    Illegal BamHI site found at 5701
    Illegal XhoI site found at 8556
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 4528
    Illegal suffix found in sequence at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 4528
    Illegal XbaI site found at 4543
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal AgeI site found at 5536
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 10115
    Illegal BsaI.rc site found at 1854
    Illegal BsaI.rc site found at 7124
    Illegal SapI site found at 518
    Illegal SapI site found at 5518
    Illegal SapI.rc site found at 10440