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Plasmid

Part:BBa_K4630120:Sequence Analysis

Designed by: Zhejun Qin   Group: iGEM23_WHU-China   (2023-10-12)
Sequence analyses related to BBa_K4630120 (length: 6458bp)

J72000		f1 ori
K314110
The sequence analysis tools use a blast database that was last updated on Thu Feb 8 10:32:16 2024. (Update now)

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Sequence and Features for BBa_K4630120
Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1221
    Illegal SpeI site found at 912
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 912
    Illegal NotI site found at 165
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1287
    Illegal BamHI site found at 903
    Illegal XhoI site found at 158
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1221
    Illegal SpeI site found at 912
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1221
    Illegal SpeI site found at 912
    Illegal NgoMIV site found at 1319
    Illegal NgoMIV site found at 2907
    Illegal NgoMIV site found at 3067
    Illegal NgoMIV site found at 6118
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 3987