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Composite

Part:BBa_K4060520:Sequence Analysis

Designed by: Cheng-Ju Lu   Group: iGEM21_NYCU-Taipei   (2021-10-21)
Sequence analyses related to BBa_K4060520 (length: 5653bp)

B0010
B0012
J23110
B0030
K4060505
B0010
B0012
J23110
B0030
K4060511
B0010
B0012
J23110
B0030
I15008
B0010
B0012
K763004
The sequence analysis tools use a blast database that was last updated on Thu Feb 8 10:32:16 2024. (Update now)

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Sequence and Features for BBa_K4060520
Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 3064
    Illegal SpeI site found at 3100
    Illegal PstI site found at 963
    Illegal PstI site found at 1260
    Illegal PstI site found at 3457
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 3064
    Illegal NheI site found at 144
    Illegal NheI site found at 167
    Illegal NheI site found at 2551
    Illegal NheI site found at 2574
    Illegal NheI site found at 3965
    Illegal NheI site found at 3988
    Illegal SpeI site found at 3100
    Illegal PstI site found at 963
    Illegal PstI site found at 1260
    Illegal PstI site found at 3457
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 3064
    Illegal BamHI site found at 619
    Illegal XhoI site found at 2369
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 3064
    Illegal SpeI site found at 3100
    Illegal PstI site found at 963
    Illegal PstI site found at 1260
    Illegal PstI site found at 3457
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 3064
    Illegal SpeI site found at 3100
    Illegal PstI site found at 963
    Illegal PstI site found at 1260
    Illegal PstI site found at 3457
    Illegal NgoMIV site found at 282
    Illegal NgoMIV site found at 570
    Illegal NgoMIV site found at 605
    Illegal NgoMIV site found at 1853
    Illegal NgoMIV site found at 2355
    Illegal NgoMIV site found at 2761
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1410
    Illegal BsaI.rc site found at 2256