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Plasmid_Backbone

Part:BBa_K3579017:Sequence Analysis

Designed by: Amandine Maire   Group: iGEM20_Paris_Bettencourt   (2020-10-27)
Sequence analyses related to BBa_K3579017 (length: 6887bp)

K3579017
The sequence analysis tools use a blast database that was last updated on Thu Feb 8 10:32:16 2024. (Update now)

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Sequence and Features for BBa_K3579017
Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2379
    Illegal SpeI site found at 552
    Illegal PstI site found at 682
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2379
    Illegal NheI site found at 6610
    Illegal SpeI site found at 552
    Illegal PstI site found at 682
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2379
    Illegal BglII site found at 409
    Illegal BglII site found at 491
    Illegal BamHI site found at 537
    Illegal XhoI site found at 530
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2379
    Illegal SpeI site found at 552
    Illegal PstI site found at 682
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2379
    Illegal SpeI site found at 552
    Illegal PstI site found at 682
    Illegal NgoMIV site found at 6758
    Illegal AgeI site found at 6227
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 6877
    Illegal BsaI.rc site found at 6024
    Illegal SapI site found at 3135
    Illegal SapI site found at 3911