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Translational_Unit

Part:BBa_K2257001:Sequence Analysis

Designed by: Ruoyun Wang   Group: iGEM17_Nanjing-China   (2017-10-22)
Sequence analyses related to BBa_K2257001 (length: 3543bp)

K2257001
The sequence analysis tools use a blast database that was last updated on Thu Feb 8 10:32:16 2024. (Update now)

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Sequence and Features for BBa_K2257001
Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 2049
    Illegal suffix found in sequence at 1
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2049
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 2055
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2049
    Illegal BglII site found at 3526
    Illegal BamHI site found at 2071
    Illegal XhoI site found at 1033
    Illegal XhoI site found at 1925
    Illegal XhoI site found at 2479
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 2049
    Illegal suffix found in sequence at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 2049
    Illegal XbaI site found at 2064
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NgoMIV site found at 2886
    Illegal AgeI site found at 2961
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2220