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Plasmid_Backbone

Part:BBa_J428342:Sequence Analysis

Designed by: Marcos Valenzuela-Ortega   Group: 2021 Engineering Committee   (2022-06-15)
Sequence analyses related to BBa_J428342 (length: 2984bp)

F1005
D1014		Suffix
G00001
J428301
J428305
J428302
J428308
J428303
J428304
J428300		Prefix
G00000
D1013
F1001
The sequence analysis tools use a blast database that was last updated on Thu Feb 8 10:32:16 2024. (Update now)

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Sequence and Features for BBa_J428342
Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found at 2948
    Illegal suffix found at 16
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2948
    Illegal NheI site found at 2
    Illegal SpeI site found at 17
    Illegal PstI site found at 31
    Illegal NotI site found at 24
    Illegal NotI site found at 2954
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2948
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 2948
    Illegal suffix found at 17
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 2948
    Plasmid lacks a suffix.
    Illegal XbaI site found at 2963
    Illegal SpeI site found at 17
    Illegal PstI site found at 31
    Illegal NgoMIV site found at 1390
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal SapI.rc site found at 2188