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Plasmid_Backbone

Part:BBa_J23018:Sequence Analysis

Designed by: John Anderson   Group: iGEM06_Berkeley   (2006-08-03)
Sequence analyses related to BBa_J23018 (length: 2298bp)

J23018
The sequence analysis tools use a blast database that was last updated on Thu Feb 8 10:32:16 2024. (Update now)

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InconsistentQC08 Plate 633, Well 40324meagan2008-05-16
Sample is in Well: Spring 2008 Distribution->Source Plate 1009: 1F

Sequence and Features for BBa_J23018
Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Illegal EcoRI site found at 2058
    Illegal XbaI site found at 2292
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2058
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 2064
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2058
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Illegal suffix found at 2
    Illegal EcoRI site found at 2058
    Illegal XbaI site found at 2292
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2058
    Illegal XbaI site found at 2292
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI.rc site found at 1097