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  • This part is mainly about the pink-white screening method. For more information and characterization of the carbohyd ...ue to pCons-AsPink being cleaved from the plasmid. This results in a "pink-white screening". ]]
    9 KB (1,495 words) - 14:37, 20 October 2019
  • ...rner of the video, the appearance of blue point represents the change from white light to 5W LED blue light (470nm)</p> ...pan style="color:#F00"><b>Red points</b></span> represents the track under white light. ]</p>
    15 KB (2,495 words) - 19:19, 18 September 2015
  • ...r PSII (Fig.1), as FRL PSII harvests about 3.6 times more photons than its white-light counterpart (as Chl a-only PSII does not absorb above 700 nm). ...bacterium. J. Biol. Chem. 275, 20652–20659 (2000).</ref> (designated as white-light PSII (WL PSII), black) and FRL PSII of C. fritschii (red) normalized
    17 KB (2,433 words) - 12:07, 12 October 2023
  • ...by the red color of the reporter RFP, while false-positive colonies remain white. ...aphics show that in the absent of D-alanine more false-positive phenotypic white colonies occur (cyan bars) compared to the samples with supplementation of
    37 KB (5,637 words) - 13:10, 19 October 2014
  • ...helps to distinguish between wrong colonies (green) and correct colonies (white) ...ystem (BBa_P10500) has been designed as described and can be used for blue white screening.<br>
    21 KB (3,433 words) - 17:20, 13 October 2022
  • |Standardised blue-white screening ===Blue-White Screening===
    6 KB (838 words) - 01:32, 18 October 2018
  • ...body. MW – molecular weight. The black arrow represents the MHETase, the white arrow the MUT-PETase. The expression of both MHETase (~70 kDa) and MUT-PETa ...a) and UVM4 were used as positive and negative controls, respectively. The white arrow indicates MUT-PETase. <span class="accent">(d)</span> Immunfluorescen
    28 KB (4,386 words) - 23:26, 13 December 2019
  • |rowspan="2" style="color:white; background-color:#56AD77;"|<b>super-folder GFP expression</b> |rowspan="2" style="color:white; background-color:#56AD77;"|<b>enhanced GFP expression</b>
    19 KB (2,814 words) - 02:46, 28 October 2020
  • ...den Gate cloning step. The success of cloning is thereby monitored by blue-white selection. The second gene encodes the cyclizing asparaginyl endopeptidase ...nd a reverse (rev) primer, red bars show correctly aligned base pairs, and white parts mismatches.]]
    38 KB (5,771 words) - 02:32, 22 October 2021
  • To confirm the N-filler worked as intended, we did blue-white colony screening, colony PCR of the plasmid insert, and also a simple GFP e ===Blue-white screening===
    13 KB (1,996 words) - 19:33, 7 October 2022
  • To confirm the O-filler worked as intended, we did blue-white colony screening, colony PCR of the plasmid insert, and also a simple GFP e ===Blue-white screening===
    6 KB (970 words) - 08:36, 12 October 2022
  • ...g out the ADE4 gene in an ADE2 mutant strain (such as YPH500) restores the white colony phenotype by undercutting the adenine biosynthesis pathway (Ugolini, Therefore a white colony in this background indicates successful integration at the correct l
    5 KB (792 words) - 03:18, 28 October 2010
  • ...faster and become more and more abundant. The eventual high proportion of white cells will lead to an increase of the planetary albedo effect and a decreas ...f Li and Lindquist (2000) or Tyedmers ''et al.'' (2008), the proportion of white yeasts growing in our plates was unexpectedly high. Further optimization of
    13 KB (1,991 words) - 17:47, 26 October 2017
  • ...resulting western blot. The enzyme CYP3A4 is marked by a black arrow, the white arrow marks a cross reaction of antibodies. For reference, the UVM4 recipie ...resulting western blot. The enzyme CYP3A4 is marked by a black arrow, the white arrow marks a cross reaction of antibodies. For reference, the UVM4 recipie
    11 KB (1,795 words) - 07:48, 12 October 2023
  • ...ively, served as positive controls. The black arrow points to MHETase, the white arrow to MUT-PETase. ...ures 4, served as positive control. The black arrow points to MHETase, the white arrow to MUT-PETase and the grey arrow to RPL1 (chloroplast ribosomal 50S p
    23 KB (3,693 words) - 23:57, 13 December 2019
  • ...ively, served as positive controls. The black arrow points to MHETase, the white arrow to MUT-PETase. ...ures 4, served as positive control. The black arrow points to MHETase, the white arrow to MUT-PETase and the grey arrow to RPL1 (chloroplast ribosomal 50S p
    15 KB (2,352 words) - 22:48, 13 December 2019
  • ...single cloning step, using the Type IIS restriction enzyme Esp3I and blue-white selection. ...ciently facilitated. Success of this cloning step can be monitored by blue-white selection.
    9 KB (1,303 words) - 19:19, 20 October 2021
  • <i>BTH101</i> has white colonies indicating that no visible RFP was produced. This data ...oter. A : Bacteria transformed or not with BBa_J04450 sequence, naked eye, white light. B. Bacteria without mRFP1 gene, excited at 546nm. C. Bacteria with m
    65 KB (10,350 words) - 11:26, 11 October 2023
  • ...ith a constitutive promotor to see if the protein could be used for a pink/white screening. We also tested the bindning capacity of the CBD-asPink protein t <b> Pink-white screening </b><br>
    9 KB (1,426 words) - 04:03, 12 October 2022
  • ...ut helps to distinguish between wrong colonies (red) and correct colonies (white) ...ystem (BBa_P10500) has been designed as described and can be used for blue white screening.<br>
    18 KB (2,921 words) - 10:56, 27 October 2020

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