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  • Collections/iGEM Type IIS Collections
    ==Team Uppsala 2020's iGEM Type IIS Standard collection== ...rted_Assembly_Standards”>registry standard</a></html>, and multiple Type IIS backbones and parts were present in the <html><a href=”https://parts.igem
    2 KB (321 words) - 17:22, 16 December 2020

Page text matches

  • Loop Assembly
    ...(Type IIS Golden Gate) standard, but we have not fully supported any Type IIS standard. That said, Type IIS assembly methods address some of the limitations of BioBricks while maintai
    9 KB (1,333 words) - 22:33, 30 November 2018
  • Part:BBa K2983030
    ...scarless assembly of a large number of DNA fragments. It makes use of type IIS restriction enzymes, such as BsaI, BsmBI, BbsI, SapI, etc., that have the p ====A Type IIS RFC[10] Loop assembly system for ''Yarrowia lipolytica''====
    16 KB (2,207 words) - 17:33, 21 October 2019
  • Part:BBa K2983031
    ...scarless assembly of a large number of DNA fragments. It makes use of type IIS restriction enzymes, such as BsaI, BsmBI, BbsI, SapI, etc., that have the p ====A Type IIS RFC[10] Loop assembly system for ''Yarrowia lipolytica''====
    15 KB (2,123 words) - 18:16, 21 October 2019
  • Part:BBa K2983032
    ...scarless assembly of a large number of DNA fragments. It makes use of type IIS restriction enzymes, such as BsaI, BsmBI, BbsI, SapI, etc., that have the p ====A Type IIS RFC[10] Loop assembly system for ''Yarrowia lipolytica''====
    15 KB (2,122 words) - 18:16, 21 October 2019
  • Part:BBa K2983033
    ...scarless assembly of a large number of DNA fragments. It makes use of type IIS restriction enzymes, such as BsaI, BsmBI, BbsI, SapI, etc., that have the p ====A Type IIS RFC[10] Loop assembly system for ''Yarrowia lipolytica''====
    15 KB (2,122 words) - 18:17, 21 October 2019
  • Part:BBa K2983034
    ...scarless assembly of a large number of DNA fragments. It makes use of type IIS restriction enzymes, such as BsaI, BsmBI, BbsI, SapI, etc., that have the p ====A Type IIS RFC[10] Loop assembly system for ''Yarrowia lipolytica''====
    15 KB (2,122 words) - 18:17, 21 October 2019
  • Part:BBa K2983035
    ...scarless assembly of a large number of DNA fragments. It makes use of type IIS restriction enzymes, such as BsaI, BsmBI, BbsI, SapI, etc., that have the p ====A Type IIS RFC[10] Loop assembly system for ''Yarrowia lipolytica''====
    15 KB (2,121 words) - 18:18, 21 October 2019
  • Part:BBa K2983036
    ...scarless assembly of a large number of DNA fragments. It makes use of type IIS restriction enzymes, such as BsaI, BsmBI, BbsI, SapI, etc., that have the p ====A Type IIS RFC[10] Loop assembly system for ''Yarrowia lipolytica''====
    15 KB (2,111 words) - 18:19, 21 October 2019
  • Part:BBa K2983037
    ...scarless assembly of a large number of DNA fragments. It makes use of type IIS restriction enzymes, such as BsaI, BsmBI, BbsI, SapI, etc., that have the p ====A Type IIS RFC[10] Loop assembly system for ''Yarrowia lipolytica''====
    15 KB (2,111 words) - 18:19, 21 October 2019
  • Part:BBa K2983038
    ...scarless assembly of a large number of DNA fragments. It makes use of type IIS restriction enzymes, such as BsaI, BsmBI, BbsI, SapI, etc., that have the p ====A Type IIS RFC[10] Loop assembly system for ''Yarrowia lipolytica''====
    15 KB (2,111 words) - 18:19, 21 October 2019
  • Part:BBa K2983039
    ...scarless assembly of a large number of DNA fragments. It makes use of type IIS restriction enzymes, such as BsaI, BsmBI, BbsI, SapI, etc., that have the p ====A Type IIS RFC[10] Loop assembly system for ''Yarrowia lipolytica''====
    15 KB (2,111 words) - 18:20, 21 October 2019
  • Collections/iGEM Type IIS Collections
    ==Team Uppsala 2020's iGEM Type IIS Standard collection== ...rted_Assembly_Standards”>registry standard</a></html>, and multiple Type IIS backbones and parts were present in the <html><a href=”https://parts.igem
    2 KB (321 words) - 17:22, 16 December 2020
  • Part:BBa K2983051
    ...pTef1 promoter that we'll refer to as pTef1d. It is derived from the wild type pTef1 promoter (nucleotides 1227374 to 1226969) of ''Yarrowia lipolytica'' ...ite (GGTCTC to GGTCTg) which makes this part compatible with the iGEM Type IIS RFC[1000] standard.
    15 KB (2,301 words) - 17:24, 21 October 2019
  • Part:BBa K2983053
    ...pTef1 promoter that we'll refer to as pTef1f. It is derived from the wild type pTef1 promoter (nucleotides 1227367 to 1227009) of ''Yarrowia lipolytica'' ...not have the BsaI site which makes this part compatible with the iGEM Type IIS RFC[1000] standard.
    16 KB (2,353 words) - 17:21, 21 October 2019
  • Part:BBa K2983052
    ...not have the BsaI site which makes this part compatible with the iGEM Type IIS RFC[1000] standard. ...how that this mutation, together with 4 others differences present in wild type as well as its shorter length, do not at all affect its activity when compa
    15 KB (2,294 words) - 17:15, 21 October 2019
  • Part:BBa K2983050
    ...ite (GGTCTC to GGTCTg) which makes this part compatible with the iGEM Type IIS RFC[1000] standard. ...tage: the presence of a BsaI site that makes it incompatible with the Type IIS RFC[1000]-compatible Loop assembly system that we designed for ''Y. lipolyt
    15 KB (2,282 words) - 17:23, 21 October 2019
  • Part:BBa K3122003
    ...he constitutive medium promoter, from Anderson family, was adapted to type IIS enzymes standard. <li><partinfo>BBa_K3122003</partinfo>: Promoter J23107 adapted to type IIS assembly</li>
    2 KB (308 words) - 09:42, 22 November 2019
  • Part:BBa K747000
    ...e sticky-end, which will be produced, if you digest the Direpeats with the type II restriction enzyme BSmBI. The cloning can be performed in one single res ...redefined order. In case a destination vector is added, that contains type IIs restriction sites pointing in opposite directions, the intermediate piece g
    12 KB (1,956 words) - 00:13, 4 September 2020
  • Part:BBa K747005
    ...e sticky-end, which will be produced, if you digest the Direpeats with the type II restriction enzyme BSmBI. The cloning can be performed in one single res ...redefined order. In case a destination vector is added, that contains type IIs restriction sites pointing in opposite directions, the intermediate piece g
    12 KB (1,932 words) - 19:02, 3 September 2020
  • Part:BBa K747002
    ...e sticky-end, which will be produced, if you digest the Direpeats with the type II restriction enzyme BSmBI. The cloning can be performed in one single res ...redefined order. In case a destination vector is added, that contains type IIs restriction sites pointing in opposite directions, the intermediate piece g
    12 KB (1,932 words) - 20:04, 27 August 2020

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