Search results (Hint: Try the special 'Search Parts' system on the main page)

Create the page "PASSENGER" on this wiki! See also the search results found.

  • Part:BBa K300000
    The ''passenger'' is the desired DNA part to be integrated into the genome. The ''guide'' is the DNA sequence that is used to target the ''passenger'' into a specific locus in the genome.
    5 KB (746 words) - 16:56, 25 January 2013
  • Part:BBa K3984004
    The N-terminal domain of ice crystal nucleoprotein (INP-N) can carry passenger proteins and anchor on the surface of bacterial cell membranes. ...The cell surface display system is generally composed of carrier protein, passenger protein, and recipient cells.
    11 KB (1,749 words) - 06:50, 21 October 2021
  • Part:BBa K4332001
    ...The cell surface display system is generally composed of carrier protein, passenger protein, and recipient cells. ...ent, in the field of removing environmental pollutants, researchers divide passenger proteins into two categories; one is the target protein naturally secreted
    10 KB (1,613 words) - 06:57, 11 October 2022
  • Part:BBa K108005
    In order to exert the display function, we chose to add the passenger protein to a special middle region of FlgH, and the two were fused through ...interaction between FlgH and LPS</i><sup>[2]</sup>, we chose to insert the passenger protein between T108 and V109.
    4 KB (562 words) - 12:23, 12 October 2023
  • Part:BBa K2694001
    ...-terminal beta-barrel domain, allowing the secretion of the amino-terminal passenger domain harboring the catalytic activity. EstA is an autotransporter belongi ...outer membrane. The catalytically inactivated extracellular domain and the passenger domain are subsequently translocated to the cell.
    12 KB (1,742 words) - 00:46, 14 October 2022
  • Part:BBa K5393005
    The Fap2 passenger domain is the coding sequence for the adhesin Fap2, an autotransporter prot ...ults in our experiments. As a negative control, a plasmid lacking the Fap2 passenger domain failed to express Fap2.
    3 KB (536 words) - 03:39, 2 October 2024
  • Part:BBa M10088
    ...nery, a transporter domain, to secrete its covalently attached, N-terminal passenger across the outer membrane. After secretion to the periplasm through normal ...essively into its native configuration as it reaches the cell surface. The passenger peptide remains in a non-native conformation while it is in the cytoplasm a
    2 KB (236 words) - 17:23, 11 May 2009
  • Part:BBa K346007
    ...at forms an integral outer membrane protein, also called β domain[2]. The passenger domain(α domain) confers the autoaggregation phenotype and it is bound to ...ntigen 43's coding sequence. Antigen 43 contains a signal peptide (red), a passenger domain (green), an autochaperone domain (blue) and a translocation unit (li
    6 KB (838 words) - 20:51, 4 October 2011
  • Part:BBa K811005
    scFv we used as a passenger protein to this surface expression domain was anti-Norovirus scFv. It speci ...use anti-His tag antibody to detect their surface expression regardless of passenger protein placed downstream. <br>
    5 KB (735 words) - 17:44, 19 October 2016
  • Part:BBa K1659002
    ...recently been shown that the DsbA signal sequence is capable of mediating passenger protein secretion under a selection of different induction temperatures [9] ...sbA 2-19 signal sequence works as expected in helping the secretion of the passenger Art175 moiety through the Sec export system, DsbA-Art175 should be expected
    14 KB (1,943 words) - 20:47, 18 September 2015
  • Part:BBa K1933001
    ...ing[1]. This is used as an anchoring motif for the cell surface display of passenger proteins. This domain does not have signal peptide for anchoring on the cel ...ein placed downstream. We confirmed this with detections of the INPNC-His-(passenger protein) by Western blotting using anti-His tag antibody(Fig1(a)).
    7 KB (1,133 words) - 15:21, 19 October 2016
  • Part:BBa K811005:Experience
    scFv we used as a passenger protein to this surface expression domain was anti-Norovirus scFv. It speci ...use anti-His tag antibody to detect their surface expression regardless of passenger protein placed downstream. <br>
    3 KB (471 words) - 12:31, 20 October 2016
  • Part:BBa K2485015
    ...(Robbins et al.). The IcsA protein additionally consists of two parts; the passenger domain, or the region responsible for unipolar localization, and the beta b ...ruct, we designed the coding sequence to be a conjugation between the IcsA passenger domain and GFP using 5X poly-glycine linker. A biotinylation site was encod
    27 KB (4,191 words) - 02:51, 31 October 2017
  • Part:BBa K3394006
    ...A-I auto-display system we chose, utilizes the translocation mechanism of passenger proteins across the outer membrane through a C-terminal beta-barrel and its ...was mutated in order to obtain a stable surface display of the recombinant passenger proteins.
    2 KB (273 words) - 17:04, 25 October 2020
  • Part:BBa K3788011
    ..., with a mutation that permit to have no protein cleavage, and keep it the passenger protein attached to the extracellular membrane.</p> ...ence that forms a pore in the external membrane and a linker that allows a passenger protein to go through the beta barrel to join the extracellular medium.</p>
    1 KB (186 words) - 15:52, 21 October 2021
  • Part:BBa K5302014
    ...d an extracellular region at the C terminal or a "passenger" domain. This "passenger" domain is linked to the β barrel domain by a short linker sequence. ...ompartment of the N terminal β barrel located in the outer membrane. The "passenger" domain of intimin forms a rod-like extension, consisting of four tandem ba
    2 KB (338 words) - 13:40, 1 October 2024
  • Part:BBa K300000:Experience
    |'''Passenger''' ...ive controls. Primer pairs VF2/VR and P1/P4 were used to validate if the ''passenger'' of interest was still present in the genome and the length of the entire
    25 KB (3,992 words) - 17:39, 25 January 2013
  • Part:BBa K633001
    ...fusion proteins to an inactive variant of EstA has already been described. Passenger enzymes in that fusion retain their hydrolytic activities after being ancho ...iew of EstA from the side, with the β-barrel domain colored green and the passenger domain
    3 KB (452 words) - 00:38, 20 October 2011
  • Part:BBa K1659301
    ...recently been shown that the DsbA signal sequence is capable of mediating passenger protein secretion under a selection of different induction temperatures [16 ...003. The DsbA signal sequence directs efficient, cotranslational export of passenger proteins to the Escherichia coli periplasm via the signal recognition parti
    9 KB (1,251 words) - 01:17, 19 September 2015
  • Part:BBa K1659201
    ...recently been shown that the DsbA signal sequence is capable of mediating passenger protein secretion under a selection of different induction temperatures [12 ...003. The DsbA signal sequence directs efficient, cotranslational export of passenger proteins to the Escherichia coli periplasm via the signal recognition parti
    7 KB (927 words) - 08:41, 14 September 2015

View (previous 20 | next 20) (20 | 50 | 100 | 250 | 500)