Part:BBa_K3788011

NOTOC Aida-I (D33:N) T5SS

BBa_K3788011 is coding for AidaA-I (D>N). It is an autotransporter from the T5SS; it allow the addressing to the extracellular medium, with a mutation that permit to have no protein cleavage, and keep it the passenger protein attached to the extracellular membrane.

The sequence is coding for the beta-barrel sequence that forms a pore in the external membrane and a linker that allows a passenger protein to go through the beta barrel to join the extracellular medium.

Wild-Type Aida-I has an autolytic activity that allows the passenger protein cleavage from the rest of the complex so it is released in the extracellular medium, but we introduced D:33N substitution the the cleavage site is not recognized anymore and the passenger protein remains attached to the cell.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 1084
  
• 1000
  COMPATIBLE WITH RFC[1000]