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- This part is made up of the basic parts: Leucine zipper Z1 (<a href="https://parts.igem.org/P ...iGEM team to use ELPs for the purpose of formation of a hydrogel and have made many different parts while investigating the possibilities. This part turne51 KB (8,206 words) - 13:09, 12 October 2023
- ...ing correct assemblies by selecting fluorescent colonies, a colony PCR was made, where the appurtenant gel can be seen in figure 2. The primers for the PCR An experiment was made to assess the response of the riboswitch at a range of different fluoride c22 KB (3,444 words) - 15:56, 12 October 2023
- ...Growth was at 37°C with shaking over 6h. Error bars are SEM, n=4. Figure made by Imperial College London 2013 iGEM. ...Growth was at 37°C with shaking over 6h. Error bars are SEM, n=4. Figure made by Imperial College London 2013 iGEM.22 KB (3,486 words) - 01:22, 22 October 2019
- <h1>Contribution made by Stockholm 2022</h1> <h1>Documentation made by ICJFLS 2021</h1>9 KB (1,482 words) - 14:45, 12 October 2022
- ...7 promoter. These plasmids have Kanamycin resistance, so the cultures were made using 5 mL of LB medium with 5 uL of 50 ug/uL Kanamycin and grown overnight 2) 1:10 dilutions of cells to LB medium were made by adding 100 uL of cells to 900 uL of LB with 1 uL of Kanamycin. From this22 KB (3,528 words) - 00:24, 11 October 2014
- ...made out of E. Coli K12 strain and stocked in the BioE44 F13 box. They are made for the testing of our pH sensor. Glycerol stock of the positive control (E.coli with p82 plasmid made before): 013301030112 KB (1,299 words) - 12:29, 7 December 2013
- ...Growth was at 37°C with shaking over 6h. Error bars are SEM, n=4. Figure made by Imperial College London 2013 iGEM. ...Growth was at 37°C with shaking over 6h. Error bars are SEM, n=4. Figure made by Imperial College London 2013 iGEM.8 KB (1,391 words) - 12:53, 3 October 2013
- ...an the BBa_J23106 part, producing roughly 13x more RFP than the BBa_J23100 made of the mNeonGreen. ...t collection] of parts compatible with the Golden Gate assembly method, we made the part [https://parts.igem.org/Part:BBa_K2656004 BBa_K2656004] which is t28 KB (4,370 words) - 17:12, 26 October 2020
- ...a bacterial stock of our own, coming from commercial bacteria cultured and made competent using our protocol <a href="http://2018.igem.org/Team:Grenoble-Al We made an heat-shock transformation <a href="http://2018.igem.org/Team:Grenoble-Al65 KB (10,350 words) - 11:26, 11 October 2023
- .... eutropha cells, P(3HB) is made through 3 steps. Two acetyl-CoA molecules made from carbohydrate converted to acetoacetyl-CoA by acetyl-CoA acetyltransfer ...Growth was at 37°C with shaking over 6h. Error bars are SEM, n=4. Figure made by Imperial College London 2013 iGEM.10 KB (1,571 words) - 13:10, 16 October 2014
- .... eutropha cells, P(3HB) is made through 3 steps. Two acetyl-CoA molecules made from carbohydrate converted to acetoacetyl-CoA by acetyl-CoA acetyltransfer ...Growth was at 37°C with shaking over 6h. Error bars are SEM, n=4. Figure made by Imperial College London 2013 iGEM.13 KB (1,995 words) - 00:38, 4 September 2020
- ...a bacterial stock of our own, coming from commercial bacteria cultured and made competent using our protocol <a href="http://2018.igem.org/Team:Grenoble-Al We made an heat-shock transformation <a href="http://2018.igem.org/Team:Grenoble-Al44 KB (7,083 words) - 21:07, 27 September 2019
- ...ere identified. After measuring activity of BPUL a successful scale up was made up to 3 L and also up to 6 L that enables an intense screening af After the measurement of BPUL activity we made a scale-up and fermented ''E. coli'' KRX with <partinfo>BBa_K863000</p56 KB (8,507 words) - 03:29, 22 October 2019
- </a> of iGEM2014 and made [BBa_K1859015]. ...er, we made the device which express circular mRNA by using this part, and made its express in <i>E. coli</i>. Then, complementary sequences are included i6 KB (1,053 words) - 03:39, 25 September 2015
- </a> of iGEM2014 and made [BBa_K1859016]. ...er, we made the device which express circular mRNA by using this part, and made its express in <i>E. coli</i>. Then, complementary sequences are included i6 KB (1,055 words) - 03:30, 25 September 2015
- , and made ...er, we made the device which express circular mRNA by using this part, and made its express in <i>E. coli</i>. Then, complementary sequences are included i4 KB (657 words) - 02:56, 25 September 2015
- ...dialyse the proteins to remove imidazole, since the dialysis membrane was made of cellulose, and our fusion proteins would all bind to the membrane via th ...ogels with those biofilms. However, we saw that the GFP biofilm (fig 10.B) made some crystals and cubic structures so that the intended biofilm was less ho22 KB (3,475 words) - 15:17, 12 October 2022
- ...ere identified. After measuring activity of BPUL a successful scale up was made up to 3 L and also up to 6 L that enables an intense screening af After the measurement of BPUL activity we made a scale-up and fermented ''E. coli'' KRX with <partinfo>BBa_K863000</p57 KB (8,638 words) - 03:38, 30 September 2024
- ...s that AlphaFold3 includes. Nevertheless, this shows that the improvements made this year mark a significant step forward, paving the way for more refined ...the Gamma variant we ordered the gene fragment, encoding this variant. We made sure to include the correct restriction sites.30 KB (4,558 words) - 18:15, 1 October 2024
- <p>An ANOVA was made to see if there was a time effect between the two populations. We obtained <p>An ANOVA test was made to see if there was a time effect between the two populations. A p-value of27 KB (4,267 words) - 13:39, 1 October 2024