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  • ...was then tested with both the linear probes mechanism as well as the split Lettuce mechanism. Both mechanisms were also tested with the complement of the midd ...A fluorescent aptamer Lettuce left (<partinfo>BBa_K4245134</partinfo>) and Lettuce right (<partinfo>BBa_K4245135</partinfo>):
    14 KB (2,075 words) - 14:51, 12 October 2023
  • The Lettuce split aptamer has been tested with the complement sequence of the middle se ...middle sequence would allow for successful hybridization between the split Lettuce and RCP.
    4 KB (579 words) - 14:28, 12 October 2023
  • The Lettuce split aptamer has been tested with the complement sequence of the middle se ...middle sequence would allow for successful hybridization between the split Lettuce and RCP.
    4 KB (575 words) - 14:42, 12 October 2023
  • ===Lettuce Aptamer=== ...the split lettuce aptamer. DFHBI-1T and the lettuce right and the modified lettuce left was added to the RCP, and the fluorescence was read on the plate reade
    10 KB (1,573 words) - 12:39, 12 October 2023
  • ...the split lettuce aptamer. DFHBI-1T and the lettuce right and the modified lettuce left was added to the RCP, and the fluorescence was read on the plate reade [[File:Lettuce.png|thumb|center|500px|<i>Figure 2. Graph of split Lettuce reaction with RCP. The values represent the change in fluorescence before a
    10 KB (1,565 words) - 12:13, 12 October 2023
  • ...the split lettuce aptamer. DFHBI-1T and the lettuce right and the modified lettuce left was added to the RCP, and the fluorescence was read on the plate reade [[File:Lettuce.png|thumb|center|500px|<i>Figure 2. Graph of split Lettuce reaction with RCP. The values represent the change in fluorescence before a
    10 KB (1,567 words) - 12:42, 12 October 2023
  • ===BBa_K4245200 Produced RCP with Lettuce Aptamer=== ...the split lettuce aptamer. DFHBI-1T and the lettuce right and the modified lettuce left was added to the RCP, and the fluorescence was read on the plate reade
    8 KB (1,309 words) - 13:00, 12 October 2023
  • The Lettuce DNA Aptamer has been tested with DFHBI-1T. The Method adopted by researcher ...nt aptamer, so Lambert iGEM continued with the design and use of the split Lettuce aptamer to better fit the reporting mechanism used in conjunction with Roll
    3 KB (487 words) - 12:57, 12 October 2023
  • ...a 42 nucleotide long DNA aptamer that, when brought in close proximity to Lettuce right (<partinfo>BBa_K4245135</partinfo>), induces fluorescence of a GFP fl The mechanism in which the split Lettuce aptamer works is represented in Figure 1.
    1 KB (205 words) - 16:00, 11 October 2022
  • ...RCA Padlock Probe (<partinfo>BBa_K4245200</partinfo>). The products, split Lettuce, and DFHBI-1T were mixed and heated at 70°C for 5 minutes, then cooled and ...: Lettuce without G RCP.png|thumb|center|500px|<i>Figure 1. Graph of split Lettuce reaction with RCP. The values represent the change in fluorescence before a
    1 KB (208 words) - 17:00, 13 October 2022
  • ...a 43 nucleotide long DNA aptamer that, when brought in close proximity to Lettuce right (<partinfo>BBa_K4245135</partinfo>), induces fluorescence of a GFP fl The mechanism in which the split Lettuce aptamer works is represented in Figure 1.
    1 KB (190 words) - 15:58, 11 October 2022
  • ...a 26 nucleotide long DNA aptamer that, when brought in close proximity to Lettuce left (<partinfo>BBa_K4245134</partinfo>), induces fluorescence of a GFP flu The mechanism in which the split Lettuce aptamer works is represented in Figure 1.
    1 KB (189 words) - 16:00, 11 October 2022
  • ...alf of the middle sequence complement within the RCP would bring the split Lettuce together, thus allowing it to induce fluorescence of a GFP fluorophore mimi
    2 KB (325 words) - 19:35, 11 October 2022
  • ...ithin the Rolling Circle Product (RCP)— was added to the 3’ end of the Lettuce left sequence. ...e to include one more nucleotide complementary to the target. The modified Lettuce left (<partinfo>BBa_K4245134</partinfo>) was more functional.
    924 B (129 words) - 16:39, 13 October 2022
  • ...partinfo>BBa_K4683000</partinfo> so that during the amplification process, Lettuce Aptamers would be produced (see fig. 1). The eRCP was also tested with DFHBI-1T dye as the RCP would consist of Lettuce Aptamer sequences. The fluorescence was read on the plate reader (see fig.
    4 KB (634 words) - 12:16, 12 October 2023
  • ...scent DNA aptamer Lettuce, developed by the Weill-Cornell Medical College. Lettuce is a 99 nucleotide long DNA aptamer that induces fluorescence of a GFP fluo ...ence (VarnBuhler et al., 2022). The fluorescence-inducing main sequence of Lettuce is kept the same while flanking sequences are added to the left and right c
    1 KB (209 words) - 01:31, 11 October 2022
  • ...ss creating short sequences of DNA, with this part as the middle sequence, Lettuce aptamers can be produced and quantified (Li et al., 2017).
    2 KB (251 words) - 01:58, 11 October 2023
  • ...ithin the Rolling Circle Product (RCP)— was added to the 3’ end of the Lettuce left sequence.
    662 B (91 words) - 01:36, 11 October 2022
  • ...ment within the Rolling Circle Product— was added to the 5’ end of the Lettuce right sequence.
    615 B (84 words) - 01:38, 11 October 2022
  • The eRCP was also tested with DFHBI-1T dye as the RCP would consist of Lettuce Aptamer sequences. The fluorescence was read on the plate reader (see fig. ...eRCP+dye was significantly greater than the controls, which suggests that Lettuce aptamers were produced. According to these results, eRCA was successful.
    2 KB (368 words) - 12:15, 12 October 2023

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