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  • Part:BBa K4140008
    ...l of phenylalanine is detected via exported extracellularly via tagging by kp-sp to bin with its substrate (X-gal). === Improvement by KP-SP tagging ===
    8 KB (1,259 words) - 23:30, 11 October 2022
  • Part:BBa E0033
    === Improvement by KP-SP tagging by AFCM-Egypt 2022 team === ...ide signal that controls the secretion of B galactosidase extracellularly (KP-SP tagging) in an effort to improve the cleavage capacity of the X gal, hei
    6 KB (1,000 words) - 22:42, 11 October 2022
  • Part:BBa K4183005
    Addition of kp-sp signal peptide to the N-terminal end of GFP allows it to be secreted ext We added a signal peptide (kp-sp, BBa_K2981006) from Actinobacillus at the N-terminal end of GFP (BBa_E00
    1 KB (173 words) - 14:34, 11 October 2022
  • Part:BBa K4140007
    KP-SP is a secreted signal peptide at the N-terminus of the reporter protein t ...high levels of phenylalanine from intracellular to extracellular we linked Kp Sp signal at the N-terminus of the PAH for transmitting it extracellularly
    3 KB (400 words) - 18:53, 11 October 2022
  • Part:BBa K2981007
    The secreting peptide Kp-SP was added to the N-terminus of the pigment protein - amilCP, and realize ...200 rpm. The control group was an amilCP without the fusion signal peptide Kp-Sp. 15 ml of the bacterial solution was taken from each group and centrifug
    1 KB (208 words) - 14:31, 19 October 2019
  • Part:BBa K4183003
    (A) When the N-terminal end of lacZ did not contain kp-sp, no disassembly of X-Gal was clearly observed around the monoclonal colo (B) When the N-terminal end of lacZ contains kp-sp, X-Gal is clearly observed to be disassembled around the monoclonal colo
    2 KB (296 words) - 14:43, 11 October 2022
  • Part:BBa K4140009
    ...latory switch. PAH has been improved by our team members to be tagged with KP-sp peptides to be exported extracellularly shown in figure 1. ...ng|thumb|right|Figure(1) Shows an SBOL demonstrating PAH Improvement using Kp-Sp tagging ]]
    5 KB (741 words) - 18:55, 11 October 2022
  • Part:BBa K4907002
    ====Kp-SP==== Kp-SP is a signal peptide found in <i>Kocuria</i> sp.3-3, which guides target
    3 KB (475 words) - 09:13, 12 October 2023
  • Part:BBa K5177000:Design
    [7] Ueki T, Walker DJF, Woodard TL, Nevin KP, Nonnenmann SS, Lovley DR. An Escherichia coli Chassis for Production of El [8] Nevin KP, Zhang P, Franks AE, Woodard TL, Lovley DR. Anaerobes unleashed: Aerobic fu
    4 KB (497 words) - 17:46, 30 September 2024
  • Part:BBa K5177001:Design
    [10] Ueki T, Walker DJF, Woodard TL, Nevin KP, Nonnenmann SS, Lovley DR. An Escherichia coli Chassis for Production of El [11] Nevin KP, Zhang P, Franks AE, Woodard TL, Lovley DR. Anaerobes unleashed: Aerobic fu
    5 KB (601 words) - 19:00, 30 September 2024
  • Part:BBa K3552000
    <br>[5] Ueki T, Walker DJF, Woodard TL, Nevin KP, Nonnenmann SS, Lovley DR. An <i>Escherichia coli</i> Chassis for Productio <br>[6] Nevin KP, Zhang P, Franks AE, Woodard TL, Lovley DR. Anaerobes unleashed: Aerobic fu
    12 KB (1,726 words) - 23:38, 1 October 2024
  • Part:BBa K5177000
    [7] Ueki T, Walker DJF, Woodard TL, Nevin KP, Nonnenmann SS, Lovley DR. An Escherichia coli Chassis for Production of El [8] Nevin KP, Zhang P, Franks AE, Woodard TL, Lovley DR. Anaerobes unleashed: Aerobic fu
    13 KB (1,876 words) - 10:16, 2 October 2024
  • Part:BBa K5177001
    [10] Ueki T, Walker DJF, Woodard TL, Nevin KP, Nonnenmann SS, Lovley DR. An Escherichia coli Chassis for Production of El [11] Nevin KP, Zhang P, Franks AE, Woodard TL, Lovley DR. Anaerobes unleashed: Aerobic fu
    13 KB (1,987 words) - 10:29, 2 October 2024
  • Part:BBa K1115008:Experience
    ...coli cells were harvested at OD600=0.4, pelleted and washed three times in KP buffer. Cells were resuspended in 2mM chloroacetate or 1,2-DCA and incubat
    3 KB (376 words) - 20:13, 27 September 2013
  • Part:BBa K4140024
    ...when cleaved, is used to detect the alpha-complementation. It's linked to KP-SP ISA secreting peptide signal at the N-terminus of the reporter protein t ...ophoresis as it is in lane 3. The running part (ordered from IDT) included KP-SP and lacZ alpha.
    3 KB (387 words) - 04:41, 12 October 2022
  • Part:BBa K4140025
    ...oduce tyrosine. PAH is a hydroxylase that needs biopterin. It's linked to KP-SP ISA secreting peptide signal at the N-terminus of the protein to transm ...it is in lane 10 (the last one). The run part (ordered from IDT) included KP-SP - PAH.
    2 KB (361 words) - 04:45, 12 October 2022
  • Part:BBa K200008
    ...it is in lane 10 (the last one). The run part (ordered from IDT) included KP-SP - PAH.
    6 KB (943 words) - 19:00, 10 October 2022
  • Part:BBa K239009
    <p>[3] Hillman JD, Johnson KP, Yaphe BI. Isolation of a Streptococcus mutans strain producing a novel bac
    4 KB (584 words) - 23:01, 18 October 2016
  • Part:BBa E1010:Experience
    ..., but good to keep note of it especially in analyzing sequencing results. (KP of siGEM)
    27 KB (4,507 words) - 00:42, 21 October 2019
  • Part:BBa K592009
    The N-terminus of this protein was added with a secreted peptide (Kp-Sp,<html><a href='https://parts.igem.org/Part:BBa_K2981006'>BBa_K2981006</a
    28 KB (4,308 words) - 19:28, 21 October 2021

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