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- <h1>FRET-Acceptor: TetR-mScarlet-I</h1> ...part is a fusion protein of TetR and the red fluorescent protein mScarlet-I. It was used as a FRET31 KB (4,075 words) - 13:19, 2 October 2024
- ...proteins called cadherins. (Alberts B. Molecular Biology of the Cell. 6th ed. New York: Garland Science; 2015) ...in the extracellular space. (Alberts B. Molecular Biology of the Cell. 6th ed. Figure 19-6 New York: Garland Science; 2015)]]68 KB (7,251 words) - 16:45, 31 October 2017
- ...YNB (Yeast Nitrogen Base)). To induce the GTH1 promoter, we added 0.005% (i.e., 0.05 g/L) glucose and incubated the cultures at 28°C for 48 hours to p ..., YASARA with the FoldX plugin, and PyMOL were utilized as modeling tools. I-TASSER predicts the 3D coordinates of protein models based on sequence-to-s46 KB (6,635 words) - 06:01, 1 October 2024
- ...l was first discovered in an isolate from <i>Streptomyces venezuelae</i>, ...rase (CAT) is an enzyme originally identified in <i>Escherichia coli</i>, that mediates resistance to chloramphenicol. (Shaw 1983) CAT covalently a58 KB (7,552 words) - 03:38, 22 October 2019
- ...l was first discovered in an isolate from <i>Streptomyces venezuelae</i>, ...rase (CAT) is an enzyme originally identified in <i>Escherichia coli</i>, that mediates resistance to chloramphenicol. (Shaw 1983) CAT covalently a58 KB (7,551 words) - 03:40, 22 October 2019
- ...sphates (dNTPs) as substrates, forming inorganic pyrophosphate (<em>PP<sub>i</sub></em>) as a by-product. It plays a key role in diversifying the human ...gonucleotide<sub>n</sub> + dNTP → Oligonucleotide<sub>n+1</sub> + PP<sub>i</sub>33 KB (4,485 words) - 08:53, 2 October 2024
- <i>Agrobacterium</i> mediated transformation is one of the most prolific methods for plant engi ...976<nowiki>[3]</nowiki>]</sup>). Despite their wide use, <i>Agrobacterium</i> mediated plant transformation is still only well established for a handful26 KB (3,859 words) - 11:23, 12 October 2023
- <i>Agrobacterium</i> mediated transformation is one of the most prolific methods for plant engi ...976<nowiki>[3]</nowiki>]</sup>). Despite their wide use, <i>Agrobacterium</i> mediated plant transformation is still only well established for a handful30 KB (4,508 words) - 14:17, 12 October 2023
- Our synthetic phytochelatin (EC20 - Glu-Cis) optimized to E. coli has: I) 10 cystein amino acids being 12 codons TGC and 8 codons TGT; II) 10 glutam 9. SAMBROOK, J.; RUSSEL, D.W. Molecular Cloning: a Laboratory Manual. 3rd ed. Cold Spring HarborLaboratory Press, New York 2001.9 KB (1,365 words) - 00:14, 27 October 2016
- Our synthetic phytochelatin (EC20 - Glu-Cis) optimized to E. coli has: I) 10 cystein amino acids being 12 codons TGC and 8 codons TGT; II) 10 glutam 9. SAMBROOK, J.; RUSSEL, D.W. Molecular Cloning: a Laboratory Manual. 3rd ed. Cold Spring HarborLaboratory Press, New York 2001.9 KB (1,365 words) - 00:31, 27 October 2016
- <h3> I. EnvZ/HBEGF Receptor Modelling </h3> ...t HBEGF in, we run secondary structure prediction of EnvZ via <i>NetSurfP</i><sup>2</sup>. Then we predict its topography using TOPCONS webserver<sup>3<14 KB (2,000 words) - 18:06, 20 October 2019
- ...ion of the substrate ABTS. Finally, the absorbance was measured in a Tecan i-control infinite 200 at 419 nm. ...BTS substrate. Absorbance was measured at 419 nm in the plate reader Tecan i-control infinite 200.56 KB (8,191 words) - 03:28, 28 October 2020
- ...ion of the substrate ABTS. Finally, the absorbance was measured in a Tecan i-control infinite 200 at 419 nm. ...BTS substrate. Absorbance was measured at 419 nm in the plate reader Tecan i-control infinite 200.57 KB (8,247 words) - 03:32, 28 October 2020
- ...ion of the substrate ABTS. Finally, the absorbance was measured in a Tecan i-control infinite 200 at 419 nm. ...BTS substrate. Absorbance was measured at 419 nm in the plate reader Tecan i-control infinite 200.57 KB (8,249 words) - 03:33, 28 October 2020
- ...which can facilitate the secretion of the fused protein out of <i>E.coli</i>. ...der the control of the T7 promoter on the pET-28a(+) vector in <i>E. coli</i> BL21(DE3).9 KB (1,319 words) - 02:22, 2 October 2024
- ...al component of the type I secretion system (T1SS) in <i>Escherichia coli</i>, facilitating the translocation of proteins across both the inner and oute ...ed into the pET-28a expression vector and transformed into the <i>E. coli</i> BL21(DE3) strain for expression.9 KB (1,273 words) - 03:25, 2 October 2024
- ...which can facilitate the secretion of the fused protein out of <i>E.coli</i>. ...flexibility and resistance to proteases. Therefore, we selected (<i>GGGGS</i>)<sub>3</sub> flexible linker (1) as a short peptide to connect LMT and T720 KB (3,044 words) - 11:16, 2 October 2024
- ...omology/anolog '''Y ''' '''R '''ecognition '''E '''ngine V 2.0 (PHYRE2), I-TASSER servers, protein '''B '''asic '''L '''ocal '''A '''ligment ''' ...ioBrick did not exhibit a burden that was significantly greater than zero (i.e., it appears to have little to no impact on growth). Therefore, users can5 KB (721 words) - 04:03, 26 August 2020
- ..., Reznikoff, W. S., Riley, M., Schaechter, M.,andUmbarger, H. E., eds) 2nd Ed., pp. 123–145, American SocietyforMicrobiology, Washington, D. C. <br>[9]Yamaguchi, S.,Fujita, H., Ishihara, A., Aizawa, S.-I., andMacnab, R. M. (1986) J. Bacteriol. 166, 187–1937 KB (962 words) - 09:00, 21 September 2015
- ..., Reznikoff, W. S., Riley, M., Schaechter, M.,andUmbarger, H. E., eds) 2nd Ed., pp. 123–145, American SocietyforMicrobiology, Washington, D. C. <br>[9]Yamaguchi, S.,Fujita, H., Ishihara, A., Aizawa, S.-I., andMacnab, R. M. (1986) J. Bacteriol. 166, 187–1937 KB (1,042 words) - 08:59, 21 September 2015