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• Part:BBa K1355001
  ...sequently, messengers RNA MerPT will not be transcript. In the presence of Hg ²⁺ ,MerR protein binds to this element and dissociates from the The experiment to quantify GFP expression induced by Hg was made according to the protocol “Quantification of Green Fluorescent P
  13 KB (1,937 words) - 16:05, 1 November 2014
• Part:BBa K1420000
  ...converting it to its less toxic ionic form, Hg(II), and then into volatile Hg(0). While variations in the operon exist, with not all genes being present ...less toxic and less mobile Hg(II) which is then completely volatilized to Hg(0) by MerA. ¹</p>
  15 KB (2,408 words) - 14:21, 4 June 2015
• Part:BBa K1420001
  <p>'''•''' MerA reduces Hg(II) to Hg(0) as a means of detoxification.</p> ...ury ions into the cell, MerA plays an important role detoxifying Hg(II) to Hg(0).
  11 KB (1,771 words) - 18:56, 23 October 2020
• Part:BBa K1355003
  We designed a biobrick device to bind and inactivate Hg. It is composed by two parts: Essential biobrick (BBa_K1355001) attached to Mercury Bacter Hg bioaccumulator (DH5-alpha transformed with BBa_K1355003)
  6 KB (946 words) - 15:55, 1 November 2014
• Part:BBa K4768000
  ...Duke 2021. The aim was to utilize the oncometabolite 2-Hydroxyglutarate (2-HG), to lift DhdR-mediated gene repression, thereby initiating an <i>in situ</ ...<i><b>Figure 2: Operating principle of the biosensor.</b> In presence of 2-HG, repression is removed and the gene is expressed leading to the production
  20 KB (2,891 words) - 21:44, 10 October 2023
• Part:BBa K1420002
  ...ic and less mobile Hg²⁺ which is then completely volatilized to Hg⁰ by the enzyme MerA.</p> ...ms for mercury resistance are prevelant in nature. Resistance to inorganic Hg(II) is conferred by MerA (narrow spectrum) while additional resistance to o
  15 KB (2,311 words) - 18:56, 23 October 2020
• Part:BBa K1420004
  ...The op-merR (BBa_K5068003) is a transcriptional regulator responsive to Hg (II) (Fig. 1). The MerR family of metal regulatory factors has been shown t ...rystal Structure of the Transcription Activator Tn501 MerR in Complex with Hg (II)">
  12 KB (1,831 words) - 01:18, 29 September 2024
• Part:BBa K1420003
  ...It is a non-essential transporter, due to similar activity of MerT brining Hg(II) into the cell.</p> ...th mercury(II) chloride show the function of MerT and MerP in transporting Hg(II) into the cell..</p>
  5 KB (830 words) - 03:40, 18 October 2014
• Part:BBa K1355004
  ...)! The beautiful MerA is responsible for reduction from Hg²⁺ in Hg⁰, which is volatile at room temperature and able to passively le Figure 1: Mercury Bacter Hg bioremediator (DH5-alpha transformed with BBa_K1355004)
  6 KB (876 words) - 15:56, 1 November 2014
• Part:BBa K1420005
  ...''' MerT is a 116 kDa transmembrane protein that assists in transporting Hg(II) into the cytoplasm of the bacterial cell. </p> <p>'''•''' Accomplishes transport of Hg(II) by interactions of cysteine residues along the protein and folding into
  9 KB (1,322 words) - 10:32, 20 October 2020
• Part:BBa K1355000
  ...reductant. Transforms mercury Hg2+ in Hg0, which volatile, lipid-soluble Hg(0) diffuses through the cell membranes without the need for any dedicated e ...h quantification of mercury resistant Escherichia coli DH5α at differents Hg concentrations by Optical Density”.
  6 KB (823 words) - 15:44, 1 November 2014
• Part:BBa K4768001
  ...ject to validate our biosensor system, which includes the oncometabolite 2-HG, the DhdR protein as a gene repressor, and the <i>dhdO </i> sequence as an ...b>Figure 1: Operating principle of our biosensor.</b> In the presence of 2-HG, repression is removed and the gene is expressed leading to the production
  15 KB (2,152 words) - 16:51, 10 October 2023
• Part:BBa K5068007
  ...BBa_K5068007 (p15A-op-merR-bspA-pcpS). This plasmid can be used to detect Hg. In addition, we also performed sequence optimization to improve the sensit ...(II). Upon transforming this plasmid into competent cells, a biosensor for Hg (II) detection can be established (Fig. 2). This biosensor utilizes the pro
  8 KB (1,071 words) - 10:01, 30 September 2024
• Part:BBa K3280007
  ...ing our biological circuit were more fit to survive in a medium containing Hg, and therefore prove that not only our protein was being expressed, but als ...roteins, but even without them, our part showed to work in the presence of Hg.
  8 KB (1,213 words) - 02:16, 22 October 2019
• Part:BBa K4046100
  ...associate decreased fluorescence or luminescence with lower levels of D-2-HG, which is associated with a variety of downstream metabolic impacts. For a ...t regulated by <i>dhdO</i>. Then, we wanted to show that the presence of 2-HG leads to the de-repression of that gene in PURE system.</p>
  8 KB (1,194 words) - 08:33, 11 October 2023
• Part:BBa K5068009
  ...(II). Upon transforming this plasmid into competent cells, a biosensor for Hg (II) detection can be established (Fig. 2). ...10, 20 μmol Hg²⁺ was significantly higher than that of 0 μmol Hg²⁺, indicating that RppA was expressed and the mercury detection
  6 KB (804 words) - 08:18, 29 September 2024
• Part:BBa K2123200
  ...pression and therefore the mercury resistance mechanism. In the absence of Hg, MerR is a weak repressor. ...ties are intimately connected in MerR role as regulator. C-terminal is the Hg binding site, mediated by three cysteine residues. Then, N-terminal is link
  8 KB (1,162 words) - 09:37, 22 October 2016
• Part:BBa K2123201
  ...was developed to turn available two <i>mer</i> operon enzymes, related to Hg metabolism: organomercurial lyase (MerB) and mercury reductase (MerA), as y ...to NADP+ while FAD is reduced to FADH-, then FADH- reduces ionic mercury Hg(II) to Hg0.
  7 KB (1,032 words) - 03:50, 28 October 2016
• Part:BBa K2123204
  ...bacteria specie has mercury resistance due to membrane protein that act in Hg capture. Among those we can find a lot of different metal binding peptides, ...fficiency of Metal Binding Peptide. In this project we design a system for Hg bioaccumulation by a synthetic phytochelatin anchored in the membrane prote
  9 KB (1,365 words) - 00:14, 27 October 2016
• Part:BBa K2123104
  ...bacteria specie has mercury resistance due to membrane protein that act in Hg capture. Among those we can find a lot of different metal binding peptides, ...fficiency of Metal Binding Peptide. In this project we design a system for Hg bioaccumulation by a synthetic phytochelatin anchored in the membrane prote
  9 KB (1,365 words) - 00:31, 27 October 2016