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  • Part:BBa K2865013
    ...of sarcomeres, dissolution of myofilaments, and frequent vacuoles. In both HF and AAV9-AR117 groups, a lot of mitochondria were swollen and even ruptured .... (C) There were significant rises of heart weight to body weight ratio in HF (n =3) and AAV-AR117 treated animals compared with Sham or AAV9-AR185 treat
    21 KB (3,003 words) - 08:53, 16 October 2018
  • Part:BBa K3979003
    ...r. Single digestion of the vector was done using both BamHI HF and HindIII HF as the control. Protocols were obtained from NEB double digest calculator. <li>DD : Double Digestion by BamHI HF and HindIII HF</li>
    22 KB (3,459 words) - 23:43, 20 October 2021
  • Part:BBa K2865012
    ...r heart failure gene therapy project. It is composed of 5 basic parts: the HF inducible BNP promoter (<partinfo>BBa_K2865000</partinfo>), our novel nanob ...s high selectivity for heart tissues and BNP promoter has been reported as HF inducible in a lot of studies.[[#References|[1][2]]]These data demonstrate
    5 KB (750 words) - 15:44, 16 October 2018
  • Part:BBa K4217005
    ...eft;vertical-align:middle;word-break:normal">&nbsp;&nbsp;&nbsp;<br>HindIII-HF&nbsp;&nbsp;&nbsp;</td><td style="border-color:inherit;border-style:solid;bo <p>Approximately 500ng of plasmid DNA was digested with NcoI-HF and HindIII-HF or both in order to confirm insertion of 6X-HIS-mntR into the pTrc backbone
    33 KB (4,790 words) - 14:28, 12 October 2022
  • Part:BBa K4160018
    ...mented into the Psb1C3 plasmid. Restriction enzymes EcoR1-HF(NEB) and Spe1-HF(NEB) were used. The design of sfGFP+UTR can be seen in figure 1.</p><br> ...SB1C3 (EcoR1-HF and Spe1-HF, 2050 bp), GFP and UTR + GFP(EcoR1-HF and Spe1-HF, respectively, 923 bp and 963 bp). An analyses on the plasmid pSB1C3. It is
    12 KB (1,879 words) - 14:22, 12 October 2022
  • Part:BBa K2485015
    .... We digested using NEB EcoRI-HF enzyme and NEB PstI-HF enzyme in Cutsmart-HF buffer, and then ligated using NEB T4 DNA ligase in T4 DNA Ligase Reaction ...A Recovery Kit”). For DNA assembly we used NEB EcoRI - HF and NEB PstI - HF for digestion and T4 DNA ligase for ligation, and the NEB Gibson Assembly M
    27 KB (4,191 words) - 02:51, 31 October 2017
  • Part:BBa K2865000
    ...dies, BNP promoter can be activated by a number of factors associated with HF, including endothelin (ET), angiotensin II (Ang II), mechanical strain, and
    5 KB (737 words) - 14:47, 17 October 2018
  • Part:BBa K2865009
    ...n considered as an accurate and efficient diagnosis and prognostication of HF, and the use of BNP may be a potential targeting to improve clinical outcom ...dies, BNP promoter can be activated by a number of factors associated with HF, including endothelin (ET), angiotensin II (Ang II), mechanical strain, and
    6 KB (980 words) - 14:57, 16 October 2018
  • Part:BBa K2643002
    ...ation protocol. To note that by using the combination of EcoRI-HF and KpnI-HF restriction enzymes, one of the T7 promoters is excised, which is not a pro ...BBa_J04450 plasmid and the Tn5 amplicon were restricted with enzymes EcoRI-HF and PstI (New England Biolabs). The obtained pSB1C3 backbone and Tn5 insert
    25 KB (3,734 words) - 01:16, 11 October 2018
  • Part:BBa K4191004
    ...tion of HF increases, the concentration of H+ ions also increases (because HF is a weak acid which partially dissociates in solution). An increase in the ...ure, we hope not only to use a closed system to ensure the dissociation of HF is enabled, but to also find a more accurate method than pH level for detec
    4 KB (669 words) - 08:44, 12 October 2022
  • Part:BBa K4191001
    ...tion of HF increases, the concentration of H+ ions also increases (because HF is a weak acid which partially dissociates in solution). An increase in the ...ure, we hope not only to use a closed system to ensure the dissociation of HF is enabled, but also to find a more accurate method than pH level for detec
    4 KB (663 words) - 08:25, 12 October 2022
  • Part:BBa K4191002
    ...tion of HF increases, the concentration of H+ ions also increases (because HF is a weak acid which partially dissociates in solution). An increase in the ...ure, we hope not only to use a closed system to ensure the dissociation of HF is enabled, but also to find a more accurate method than pH level for detec
    4 KB (641 words) - 08:35, 12 October 2022
  • Part:BBa K4191003
    ...tion of HF increases, the concentration of H+ ions also increases (because HF is a weak acid which partially dissociates in solution). An increase in the ...ure, we hope not only to use a closed system to ensure the dissociation of HF is enabled, but also to find a more accurate method than pH level for detec
    4 KB (647 words) - 08:41, 12 October 2022
  • Part:BBa I746909
    ...SB1C3 (EcoR1-HF and Spe1-HF, 2050 bp), GFP and UTR + GFP(EcoR1-HF and Spe1-HF, respectively, 923 bp and 963 bp). An analyses on the plasmid pSB1C3. It is
    38 KB (5,954 words) - 13:56, 11 October 2022
  • Part:BBa K1421005
    ...and EGFP(P) of the same volume. The next is digestion by SpeI-HF and PstI-HF(NEB). The product was named as pSB1C3-RpaR&I-GFP(P). ...action of plasmid, we check the results by digestion with SpeI-HF and PstI-HF(NEB). The result of DNA agarose gel electrophoresis is shown as the figure
    3 KB (520 words) - 16:33, 30 October 2014
  • Part:BBa K1421006
    ...and EGFP(P) of the same volume. The next is digestion by SpeI-HF and PstI-HF(NEB). The product was named as pSB1C3-RpaR&I-GFP(P). ...action of plasmid, we check the results by digestion with SpeI-HF and PstI-HF(NEB). The result of DNA agarose gel electrophoresis is shown as the figure
    3 KB (520 words) - 16:34, 30 October 2014
  • Part:BBa K1421007
    ...and EGFP(P) of the same volume. The next is digestion by SpeI-HF and PstI-HF(NEB). The product was named as pSB1C3-RpaR&I-GFP(P). ...action of plasmid, we check the results by digestion with SpeI-HF and PstI-HF(NEB). The result of DNA agarose gel electrophoresis is shown as the figure
    3 KB (520 words) - 16:34, 30 October 2014
  • Part:BBa K2614000:Design
    ...PstI-HF. Subsequently we digest the pSB1C3 plasmid with EcoRI-HF and PstI-HF and ligate the digested Plasmid with our digested template.
    443 B (59 words) - 22:02, 15 October 2018
  • Part:BBa K2643000
    ..., the Linker-Tn5 PCR amplicon was restricted with enzymes FseI-HF and KpnI-HF (New England Biolabs) as well as the pACYCDuet-1 plasmid, according to the ...Then, the dxCas9 PCR amplicon was restricted with enzymes NotI-HF and FseI-HF (New England Biolabs) as well as the pACYCDuet-1-Lin-Tn5 plasmid, according
    39 KB (5,803 words) - 16:18, 12 October 2018
  • Part:BBa K3271027
    ...n-optimized for expression in <i>E. coli</i> and for enhanced performance. HF Phusion Polymerase catalyzes DNA amplification in the 5' to 3' direction. T <b> Design, Cloning, Expression, and Purification of HF Phusion Polymerase for the making of DIY Gibson Assembly Kit </b><br>
    5 KB (710 words) - 19:48, 21 October 2019

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