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- ...ts catalyzing the reverse reaction. The mean ΔRFU value was normalized to zero for normalized to zero106 KB (11,833 words) - 14:02, 19 October 2021
- ...) compared to <i>ydeh</i> (deep blue) for the reduction period. Here, time zero reflects the start of induction with 1.5mM IPTG.]] <br> ...rk gray) compared to <i>ydeh</i> (black) over a 48-hour period. Here, time zero reflects the start of induction with 1.5mM IPTG.]] <br>17 KB (2,640 words) - 04:33, 17 December 2021
- ...ple) compared to <i>cyma</i> (yellow) for the reduction period. Here, time zero reflects the start of induction with 1.5mM IPTG.]] <br> ...ay) compared to <i>cyma</i> (light blue) over a 48-hour period. Here, time zero reflects the start of induction with 1.5mM IPTG.]] <br>16 KB (2,570 words) - 04:33, 17 December 2021
- ...rple) compared to <i>ribf</i> (brown) for the reduction period. Here, time zero reflects the start of induction with 1.5mM IPTG.]] <br> ...y) compared to <i>ribf</i> (deep purple) over a 48-hour period. Here, time zero reflects the start of induction with 1.5mM IPTG.]] <br>18 KB (2,902 words) - 04:34, 17 December 2021
- ...rple) compared to <i>oprf</i> (green) for the reduction period. Here, time zero reflects the start of induction with 1.5mM IPTG.]] <br> ...) compared to <i>oprf</i> (light purple) over a 48-hour period. Here, time zero reflects the start of induction with 1.5mM IPTG.]] <br>17 KB (2,786 words) - 04:31, 17 December 2021
- ...i>riboflavin cluster</i> (light blue) for the reduction period. Here, time zero reflects the start of induction with 1.5mM IPTG.]] <br> ...to <i>riboflavin cluster</i> (turquoise) over a 48-hour period. Here, time zero reflects the start of induction with 1.5mM IPTG.]] <br>18 KB (2,837 words) - 04:34, 17 December 2021
- ===This Part was Instrumental in the UManitoba_2023 PLAnet Zero Project=== ...urther experimental details with exact procedure can be foud in the PLAnet Zero <html><a href="https://2023.igem.wiki/umanitoba/results/" target="_blank">r5 KB (768 words) - 15:47, 12 October 2023
- ...splay activated/non-activated states. For some situations, we would expect zero expression of GOI without the presence of inducer. However, leakage still e ..., and Víctor de Lorenzo to design a double-bistable system which achieves zero basal expression of gene of interest in the absence of any inducer while en24 KB (3,604 words) - 22:22, 20 October 2019
- ...splay activated/non-activated states. For some situations, we would expect zero expression of GOI without the presence of inducer. However, leakage still e ..., and Víctor de Lorenzo to design a double-bistable system which achieves zero basal expression of gene of interest in the absence of any inducer while en24 KB (3,616 words) - 22:24, 20 October 2019
- ...splay activated/non-activated states. For some situations, we would expect zero expression of GOI without the presence of inducer. However, leakage still e ..., and Víctor de Lorenzo to design a double-bistable system which achieves zero basal expression of gene of interest in the absence of any inducer while en24 KB (3,681 words) - 22:26, 20 October 2019
- ...s one of several sets of our bistable system, which is designed to achieve zero basal expression in the absence of any inducer while ensuring constant tran ..., and Víctor de Lorenzo to design a double-bistable system which achieves zero basal expression of gene of interest in the absence of any inducer while en19 KB (2,772 words) - 22:56, 20 October 2019
- ...s one of several sets of our bistable system, which is designed to achieve zero basal expression in the absence of any inducer while ensuring constant tran ..., and Víctor de Lorenzo to design a double-bistable system which achieves zero basal expression of gene of interest in the absence of any inducer while en22 KB (3,336 words) - 12:43, 12 October 2023
- ...tained RBS2, a modified version of RBS1. The system is designed to achieve zero basal expression in the absence of any inducer while ensuring constant tran ..., and Víctor de Lorenzo to design a double-bistable system which achieves zero basal expression of gene of interest in the absence of any inducer while en19 KB (2,782 words) - 23:00, 20 October 2019
- ...tained RBS2, a modified version of RBS1. The system is designed to achieve zero basal expression in the absence of any inducer while ensuring constant tran ..., and Víctor de Lorenzo to design a double-bistable system which achieves zero basal expression of gene of interest in the absence of any inducer while en19 KB (2,782 words) - 23:00, 20 October 2019
- ...tained RBS2, a modified version of RBS1. The system is designed to achieve zero basal expression in the absence of any inducer while ensuring constant tran ..., and Víctor de Lorenzo to design a double-bistable system which achieves zero basal expression of gene of interest in the absence of any inducer while en19 KB (2,782 words) - 23:01, 20 October 2019
- ...tained RBS2, a modified version of RBS1. The system is designed to achieve zero basal expression in the absence of any inducer while ensuring constant tran ..., and Víctor de Lorenzo to design a double-bistable system which achieves zero basal expression of gene of interest in the absence of any inducer while en19 KB (2,782 words) - 23:01, 20 October 2019
- ...aximum</td> </tr> <tr> <td>-</td> <td>+</td> <td>zero</td> </tr> <tr> <td>-</td> <td>-</td> <td>leaky ( ...This BioBrick did not exhibit a burden that was significantly greater than zero (i.e., it appears to have little to no impact on growth). Therefore, users3 KB (461 words) - 19:09, 27 August 2020
- ...behavior (the system oscillates with the amplitude gradually decreasing to zero): At first, we will see an oscillating concentration of GOx production, and ...ecrease at t=0 (while other bacteria were at its peak). After two hours at zero level of GOx activity, the enzyme activity raised to a final value of ~0.008 KB (1,209 words) - 16:55, 21 October 2019
- ..., and Víctor de Lorenzo to design a double-bistable system which achieves zero basal expression of gene of interest in the absence of any inducer while en ...ems of both recombinase and toxin could further reinforce the insurance of zero expression of toxin.18 KB (2,733 words) - 15:10, 21 October 2019
- ..., and Víctor de Lorenzo to design a double-bistable system which achieves zero basal expression of gene of interest in the absence of any inducer while en ...ems of both recombinase and toxin could further reinforce the insurance of zero expression of toxin.18 KB (2,726 words) - 15:13, 21 October 2019