DNA

Part:BBa_K106681:Design

Designed by: Jacinto Chen   Group: iGEM08_UCSF   (2008-10-28)


3000bp Spacer


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1728
    Illegal XbaI site found at 1732
    Illegal PstI site found at 2614
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1728
    Illegal PstI site found at 2614
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1728
    Illegal BglII site found at 1064
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1728
    Illegal XbaI site found at 1732
    Illegal PstI site found at 2614
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1728
    Illegal XbaI site found at 1732
    Illegal PstI site found at 2614
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1483


Design Notes

none.


Source

PCR'ed spacer from mammalian kinase,PI3ka.

References