File list
This special page shows all uploaded files.
| Date | Name | Thumbnail | Size | Description | Versions |
|---|---|---|---|---|---|
| 23:46, 17 October 2014 | Blue white.JPG (file) |  |
670 KB | Blue white selection is used to select the new construct following cloning. White colonies indicate that the lacz has dropped out as it has been replaced by the new construct of interest. | 1 |
| 22:33, 17 October 2014 | GoldenGate overhangs.jpg (file) |  |
13 KB | Details of the overhangs used in the GoldenGate, MoClo cloning standard. | 1 |
| 15:44, 17 October 2014 | Blue white selection.JPG (file) |  |
545 KB | Spreading of XGAL and IPTG for Blue white selection of constructs. | 1 |
| 15:23, 17 October 2014 | Infiltration analysis.tif (file) | Error creating thumbnail: Invalid thumbnail parameters |
4.77 MB | We used fluorescence microscopy to analyse the leaves of our infiltrated Nicotiana Benthamiana. | 1 |
| 13:12, 16 October 2014 | Endlinkers.jpg (file) |  |
109 KB | Design of a GoldenGate level 2 cloning reaction showing the Endlinker to be used according to the number of genes within the construct. | 1 |
| 13:53, 13 October 2014 | Level 2 GoldenGate.jpg (file) |  |
44 KB | Level two cloning allows assembly of multiple, whole transcriptional units into one construct in a single step. The Bpi1I sites are convergent on each level 1 part and divergent on the level 2 acceptor, therefore will be removed by the process of cloning. | 1 |
| 13:52, 13 October 2014 | Level 1 GoldenGate.jpg (file) |  |
28 KB | Level 0 parts can be combined in a Golden Gate dig-lig reaction with the appropriate level one acceptor to create a level one construct that will usually consist of a complete transcriptional unit. The BsaI sites are convergent on each level 0 part and di | 1 |
| 13:52, 13 October 2014 | Level 0 GoldenGate.jpg (file) |  |
22 KB | Level 0 parts consist of sequences with standard overhangs that have been cloned into a level 0 acceptor using the Type IIS REN BpiI. The level 0 acceptor has two inverted BpiI sites and two inverted BsaI sites. The BpiI sites are convergent on each inser | 1 |
| 13:16, 13 October 2014 | GFP expression.jpg (file) |  |
111 KB | Nicotiana Benthamiana leaves under UV light following infiltration of agrobacterium carrying the constructs (A) 35s_GFP_ocs, (B) BS3_GFP_ocs, (C) PDF1.2_GFP_ocs. In each case GFP protein has be transcribed following activation of the promoter and is shown | 1 |
| 17:52, 10 October 2014 | Image- PDF1.2 GFP.jpg (file) |  |
3.17 MB | Nicotiana benthamiana leaves under UV light following infiltration (red circles) with Agrobacterium tumefaciens carrying plasmids encoding PDF1.2_GFP_ocs. Leaf (B) has been treated with Methyl Jasmonate whilst the control, leaf (A), was untreated. There | 1 |
| 17:38, 10 October 2014 | BS3 GFP + AvrBS3.jpg (file) |  |
2.65 MB | A Nicotiana benthamiana leaf under UV light following infiltration (red circles) with Agrobacterium tumefaciens carrying plasmids encoding BS3_GFP_ocs (B) and BS3_GFP_ocs + 35s_AvrBS3_nos (A) respectively. There is GFP expression in the area infiltrated | 1 |
| 16:19, 9 October 2014 | 35s GFP.jpg (file) |  |
1.58 MB | A Nicotiana benthamiana leaf under UV light following infiltration (red circles) with Agrobacterium tumefaciens carrying the plasmid encoding 35s Constututive Promotor (Cauliflower Mosaic Virus) + GFP Coding Sequence + Octopine Synthase Terminator (Agroba | 1 |
| 16:12, 9 October 2014 | 35s Bax plus control.jpg (file) |  |
2.22 MB | The result of an infiltration of Agrobacterium tumefaciens into Nicotiana benthamiana, showing plant cell apoptosis caused by expression of Bax. This Bax expression also gives evidence for the function of the 35S promoter which is constitutive and therefo | 1 |
| 16:06, 9 October 2014 | 35s GFP.tif (file) |  |
8.57 MB | A Nicotiana benthamiana leaf under UV light following infiltration (red circles) with Agrobacterium tumefaciens carrying the plasmid encoding 35s Constututive Promotor (Cauliflower Mosaic Virus) + GFP Coding Sequence + Octopine Synthase Terminator (Agroba | 1 |
| 15:12, 9 October 2014 | 35s Bax plus control.tif (file) | |
13.31 MB | The result of an infiltration of Agrobacterium tumefaciens into Nicotiana benthamiana, showing plant cell apoptosis caused by expression of Bax. This Bax expression also gives evidence for the function of the 35S promoter which is constitutive and therefo | 1 |
