File:35s Bax plus control.tif

35s_Bax_plus_control.tif ‎(2,672 × 2,752 pixels, file size: 13.31 MB, MIME type: image/tiff)

The result of an infiltration of Agrobacterium tumefaciens into Nicotiana benthamiana, showing plant cell apoptosis caused by expression of Bax. This Bax expression also gives evidence for the function of the 35S promoter which is constitutive and therefore causes expression of the protein without external stimuli.

Image (A) provides a control, showing a Nicotiana benthamiana leaf infiltrated (red circles) with Agrobacterium tumefaciens whilst B shows a Nicotiana benthamiana leaf infiltrated (red circles) with Agrobacterium tumefaciens carrying the plasmid encoding: 35s Constututive Promotor (Cauliflower Mosaic Virus) + Bax Coding Sequence (Mus Musculus) + Octopine Synthase Terminator (Agrobacterium tumefaciens).

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current15:12, 9 October 20142,672 × 2,752 (13.31 MB)Cara.deal (Talk | contribs)The result of an infiltration of Agrobacterium tumefaciens into Nicotiana benthamiana, showing plant cell apoptosis caused by expression of Bax. This Bax expression also gives evidence for the function of the 35S promoter which is constitutive and therefo
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Metadata

This file contains additional information, probably added from the digital camera or scanner used to create or digitize it. If the file has been modified from its original state, some details may not fully reflect the modified file.
Camera manufacturer NIKON CORPORATION
Camera model NIKON D4
Exposure time 1/125 sec (0.008)
F Number f/16
ISO speed rating 100
Date and time of data generation 11:34, 2 October 2014
Lens focal length 60 mm
Width 2,672 px
Height 2,752 px
Compression scheme LZW
Pixel composition RGB
Orientation Normal
Number of components 4
Number of rows per strip 1
Horizontal resolution 299.999 dpi
Vertical resolution 299.999 dpi
Data arrangement chunky format
Software used Adobe Photoshop Camera Raw 8.5 (Macintosh)
File change date and time 15:15, 9 October 2014
Exposure Program Manual
Exif version 2.3
Date and time of digitizing 11:34, 2 October 2014
APEX shutter speed 6.965784
APEX aperture 8
APEX exposure bias 1
Maximum land aperture 3.4 APEX (f/3.25)
Metering mode Pattern
Light source Unknown
Flash Flash did not fire
DateTimeOriginal subseconds 60
DateTimeDigitized subseconds 60
White balance Manual white balance
Digital zoom ratio 1
Focal length in 35 mm film 60 mm
Contrast Normal
Saturation Normal
Sharpness Normal
Unique image ID 0598D51458D74F7284EF76D5F6D61EBC