Part:BBa_Y00073

Y00073 is S. pombe gene SPCC126.04c http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=protein&cmd=search&term=NP_588447.1 recoded for expression in S. cerevisiae. The pombe gene is the homologue of the cerevisiae SGF73 http://db.yeastgenome.org/cgi-bin/locus.pl?locus=sgf73, a subunit of the ATP-independent chromatin remodelling complex SAGA. Deletion of this gene in cerevisiae results in a viable strain but the PAU-genes are unresponsive to hypoxic conditions. Other phenotypes may also exist from the sgf73 deletion but are undescribed. It is not clear that the recoding of the pombe gene for expression in cerevisiae will be necessary for expression, although it's worth noting that the recoded gene is only 78% identical (812/1035 positions).