Intermediate

Part:BBa_S05458:Design

Designed by: Shamlan M. S. Reshamwala   Group: Guests   (2018-10-05)


K274380:I746351 followed by Bacillus subtilis RBS-phoD gene


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 400
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

S05458a.jpeg

The 718 bp BBa_K2802000 (lane 2) and 1771 bp BioBricked Bacillus subtilis RBS+phoD gene were joined together to make 2497 bp BBa_S05459 (lane 3). Parts were digested with EcoRI and PstI.

Source

BBa_K274380, BBa_I746351 and Bacillus subtilis RBS+phoD gene.

References