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Experience: None
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Intermediate

Part:BBa_S03960:Design

Designed by: Victoria Hsiao   Group: Guests   (2008-07-07)

B0034:K137013


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 878
    Illegal EcoRI site found at 997
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 878
    Illegal EcoRI site found at 997
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 878
    Illegal EcoRI site found at 997
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 878
    Illegal EcoRI site found at 997
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 878
    Illegal EcoRI site found at 997
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This is adding a strong RBS to the L.lactis gene folC, which is part of the folate biosynthesis gene cluster.

Source

References