Composite

Part:BBa_S03640:Design

Designed by: Karmella Haynes   Group: iGEM06_Davidson   (2006-10-30)


(-1,2) HixC-pBadrev-HixC-RBS-TetF : HixC


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 35
    Illegal NheI site found at 371
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 95
    Illegal BamHI site found at 517
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 543
    Illegal NgoMIV site found at 911
    Illegal NgoMIV site found at 1071
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

One of the eight permutations of two adjacent hixC-flanked "flippable" parts: pBad and RBS-Tet. This permutation has a reversed pBad promoter followed by a forward RBS-Tet coding region.

Source

References