Regulatory
lux pR

Part:BBa_R1062:Design

Designed by: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
Drew Endy
  Group: Antiquity   (2003-01-31)


Promoter, Standard (luxR and HSL regulated -- lux pR)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This promoter is based on the Vibrio fischeri quorum sensing gene promoters. Two genes LuxI and LuxR and transcribed in opposite directions as shown below. The original sequence from which the parts BBa_R0062 and BBa_R0063 were derived is shown in the picture below.

<img src="Include File Image1.gif" width="614" height="362">

Modified to comply with BBa_S0001:

TTGACA-17N-GATACT



Source

V. fischeri

References