Part:BBa_R0078:Experience
USP-Brazil Characterization
This part has shown to somewhat work when in the context of expressing the reporter BBa_K2771020 and co-transformed with the part BBa_K2771030. In an assay in 2mL of LB in a 24-well plate, cells were grown overnight, then diluted 1:10 and grown for 1h. This was followed by induction of the system by arabinose 2.5mM to kickstart HSL production by LuxI. At evety chosen timepoint, samples were centrifuged and ressuspended in H2O. Excitation-emission wavelenghts for fluorescence measurement were 430-480 for ECFP and 500-530 for EYFP. Data in the graph is for promoter activity expressing EYFP and having this fluorescence divided by the constitutive ECFP fluorescence measurement:
Applications of BBa_R0078
User Reviews
UNIQ7ae350699196bfe6-partinfo-00000000-QINU UNIQ7ae350699196bfe6-partinfo-00000001-QINU