Generator
ampR

Part:BBa_P1002

Designed by: Reshma Shetty   Group: BE.109 Instructors   (2006-01-23)

ampicillin resistance cassette

Ampicillin resistance cassette including promoter and coding sequence. It lacks a terminator.


Usage and Biology

References

Engineering BioBrick vectors from BioBrick parts
Journal of Biological Engineering, 2008 Apr 14;2:5
Reshma Shetty, Drew Endy, Tom Knight
[http://www.jbioleng.org/content/2/1/5 URL]
[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&id=169656099 GenBank EU496092]

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[http://2017.igem.org/Team:Heidelberg, Team Heidelberg 2017] improved this part by insertion of a mutation, yielding a higher enzyme activity (see: BBa_K2398550).
(--moritz_p95 22:12, 27.10.2017 (UTC))

[edit]
Categories
//plasmidbackbone/component/selectionmarker/antibioticresistance
//direction/forward
Parameters
o_h
proteinbeta-lactamase
rbs
tagNone