Part:BBa_M50439:Experience

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Please enter how you used this part and how it worked out.

Applications of BBa_M50439

This construct can successfully be used to measure intracellular pH given by a BRET ratio in S. cerevisiae. Due to the well-characterized pH-sensitivity of Rluc8 (luciferase), we are confident that BRET readout from cpVenus (fluorophore) is indicative of intracellular pH because it was excited through Rluc8 activity.

User Reviews

UNIQfb072dc2522a992e-partinfo-00000000-QINU UNIQfb072dc2522a992e-partinfo-00000001-QINU

This plasmid was used in Bba_M50440 and the user experience is better outlined there.

Stanford Location

Plasmid name: pHlash
DNA2.0 Gene #: pD1204-NSH
Organism: S. cerevisiae
Device type: Sensor
Glycerol stock barcode: 133021752
Box label: BioE44 S18