Device

Part:BBa_M50101

Designed by: Daniel Tang   Group: Stanford BIOE44 - S11   (2017-10-19)


VPEL222 - Optogenetic Transcription Factor

VPEL222 is a light-dependent transcription factor containing a nuclear localization sequence, VP-16 transcriptional activator, and EL-222 light-sensitive DNA-binding protein. This transcription factor can localize to the nucleus and binds to specific DNA sequences (c120 repeat sites) under blue light illumination. The VP-16 region promotes transcription of genes downstream from the c120 repeats once the transcription factor is bound.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 945
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 925
    Illegal XhoI site found at 734
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 945
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 945
    Illegal NgoMIV site found at 304
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None