Composite

Part:BBa_M50084:Design

Designed by: Karen Dai   Group: Stanford BIOE44 - S11   (2017-10-19)


Yeast Serotonin Receptor Part


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 748
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 76


Design Notes

Since it was sourced from the human genome, we optimized the serotonin receptor nucleotide sequence to yeast (Saccharomyces cerevisiae).

Source

The serotonin receptor gene comes from the human genome. We took the amino acid sequence from http://www.uniprot.org/uniprot/P08908#sequences, and then simply codon optimized for yeast to generate the nucleotide sequence.

References

Nakamura, Y., Ishii, J. and Kondo, A. (2015), Applications of yeast-based signaling sensor for characterization of antagonist and analysis of site-directed mutants of the human serotonin 1A receptor. Biotechnol. Bioeng., 112: 1906–1915. doi:10.1002/bit.25597