Plasmid

Part:BBa_M50052

Designed by: Nicholas M Kuntz   Group: Stanford BIOE44 - S11   (2016-12-11)


alkB1 + alkG

Composite Part containing both alkB1 (ABO-2707) and alkG (ABO-2708) sequences for alkane degradation (BBa_M50004 and BBa_M50005). In conjunction, these enzymes should add an -OH group to an alkane of up to thirty-two carbons in size.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 900
    Illegal EcoRI site found at 1150
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 900
    Illegal EcoRI site found at 1150
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 900
    Illegal EcoRI site found at 1150
    Illegal BglII site found at 976
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 900
    Illegal EcoRI site found at 1150
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 900
    Illegal EcoRI site found at 1150
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1573
    Illegal SapI.rc site found at 643


[edit]
Categories
Parameters
None