Terminator

Part:BBa_M50046:Design

Designed by: Mark Buckup   Group: Stanford BIOE44 - S11   (2016-12-08)


SV40 polyA Terminator


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 36
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 16
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 36
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 36
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This poly-adenosine terminator will stop transcription for mammalian cells.

Source

TA Robert Chen's plasmid

References