Protein_Domain

Part:BBa_M50036:Design

Designed by: Amanda Urke   Group: Stanford BIOE44 - S11   (2016-12-11)


6 Amino Acid Fusion Protein Linker


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codon optimization software was used since we transformed our plasmid into E. coli. Also, the linker needed to be relatively short since we were already designing a long construct and needed to minimize the synthesis time given class constraints.


Source

Linker sequence was suggested and provided by our TA, Salil Bahte, and then optimized for use in E. coli.

References