Composite

Part:BBa_M50034

Designed by: Cale Lester, Raquel Freeman, Tristan Yeung   Group: Stanford BIOE44 - S11   (2016-11-01)


U10_mKO2, IPTG inducible, KAN resistant Thermosensor Construct

At elevated temperatures, the U10 thermosensor releases its hairpin structure and allows the RBS to be readily accessible to the ribosome, enabling increased expression of our desired gene. The U10 thermosensor mainly exhibits activity at approximately 37˚C. Other temperatures such as 22˚C and 30˚C show very low gene expression levels. We picked U10 because of its higher specificity as a thermosensor in our chosen temperature ranges (22˚C - 37˚C). We did not make any modifications to the thermosensor genetic sequences given by Neupert, J., Karcher, D., and Bock, R.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 62
    Illegal PstI site found at 68
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 62
    Illegal PstI site found at 68
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 49
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 62
    Illegal PstI site found at 68
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 62
    Illegal PstI site found at 68
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 659


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Parameters
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