Composite

Part:BBa_M50015:Design

Designed by: Derek Wang, Anika Naidu   Group: Stanford BIOE44 - S11   (2016-10-27)


Positive feedback cusR producer (E. coli)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 218
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 218
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 218
    Illegal AgeI site found at 44
    Illegal AgeI site found at 402
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Designed to be grafted onto EColi_Promoter_Casette with high copy number RBS and GFP


Source

RBS: BBa_B0035 https://parts.igem.org/Ribosome_Binding_Sites/Prokaryotic/Constitutive/Community_Collection cusR promoter from E.Coli K12 (250 nts before cusR protein sequence): http://microbesonline.org/cgi-bin/fetchLocus.cgi?locus=14707&disp=4 cusR from E.Coli K12 (684 nts of protein): http://microbesonline.org/cgi-bin/fetchLocus.cgi?locus=14707&disp=4

References