Device

Part:BBa_M47008:Design

Designed by: Julian Alverio   Group: MIT BE 20.20 - S15   (2015-05-13)


Time-Delay Input Filter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1562
    Illegal BamHI site found at 2476
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 845
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Strength of interaction between the homodimerizing inducer and its promoter will determine how long the output lags the input. Multiple combinations will likely need to be tested in order to achieve desired performance.


Source

Lac Operon Promoter from E. Coli Genome Hat4 gene and COX5B-1 promoter sequence from Arabidopsis thalian genome GFP from Aequorea victoria genome

References