Coding

Part:BBa_M45642:Design

Designed by: Hossein Jahromi   Group: UtahState_BE5930_-_S14   (2015-04-13)


dnaK chaperone Hsp70


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 727
    Illegal PstI site found at 860
    Illegal PstI site found at 970
    Illegal PstI site found at 1321
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 727
    Illegal PstI site found at 860
    Illegal PstI site found at 970
    Illegal PstI site found at 1321
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 727
    Illegal BglII site found at 930
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 727
    Illegal PstI site found at 860
    Illegal PstI site found at 970
    Illegal PstI site found at 1321
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 727
    Illegal PstI site found at 860
    Illegal PstI site found at 970
    Illegal PstI site found at 1321
    Illegal NgoMIV site found at 156
    Illegal NgoMIV site found at 1671
    Illegal AgeI site found at 399
    Illegal AgeI site found at 871
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1588


Design Notes

no design considerations


Source

dnaK chaperone Hsp70, with co-chaperone DnaJ (Escherichia coli str. K-12 substr. MG1655)

References