Coding

Part:BBa_M45157:Design

Designed by: Cynthia Hanson   Group: UtahState_BE5930_-_S14   (2016-04-20)


Amino acid sequence to produce MPT64


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 456
    Illegal BamHI site found at 546
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 538
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 519


Design Notes

The amino acid sequence has been changed to be E.coli-favorable, and the restriction enzyme sites for EcoRI, PstI, SpeI, and XbaI have been removed.


Source

original amino acid sequence was taken from M. tuberculosis.

References