Reporter

Part:BBa_M45117:Design

Designed by: Federico C. Rodriguez   Group: UtahState_BE5930_-_S14   (2014-04-19)

Uranium Inducible GFPuv Reporter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1520
    Illegal XhoI site found at 1421
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2
    Illegal NgoMIV site found at 317
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 630
    Illegal SapI.rc site found at 584


Design Notes

The complete sequence complied with RFC-10 assembly compatibility as a requirement for Utah State University Synthetic Biology class, so no design considerations had to be taken into account during the creation of the sequence as a biobrick.


Source

This composite part comes from BBa_M45113 + BBa_B0034 + BBa_M45116 + BBa_B0010 + BBa_B0012.

References