Composite

Part:BBa_M45006:Design

Designed by: Charles Barentine   Group: UtahState_BE5930_-_S14   (2014-04-21)


Hairpin designed to silence canine F1 allergen


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 840
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1067
    Illegal BsaI.rc site found at 1043


Design Notes

The sense and antisense portions must properly form a loop structure in order to induce gene silencing.


Source

This composite part consists of a coding region for a hairpin loop that codes for the canine F1 allergen and the hly gene coding for listeriolysin O, derived from the bacterium Listeria monocytogenes.

References