Device

Part:BBa_M39412:Design

Designed by: Zach Banks   Group: MIT BE 20.20-S12   (2012-05-15)


DNAseI generator inhibited by recombinant pCat promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

There is a terminator before the pCat promoter to prevent "backwards" expression, which would be detrimental to the strong "digital" behavior the recombinant region attempts to achieve.

    • Everything before the fimE IRL should be on the antisense strand, and thus inverted.


Source

References