Protein_Domain

Part:BBa_M39201:Design

Designed by: Hannah Kempton   Group: MIT BE 20.20-S12   (2012-05-15)

Micro-Dystrophin


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2161
    Illegal PstI site found at 2920
    Illegal PstI site found at 3184
    Illegal PstI site found at 3283
    Illegal PstI site found at 3749
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2161
    Illegal NheI site found at 1308
    Illegal PstI site found at 2920
    Illegal PstI site found at 3184
    Illegal PstI site found at 3283
    Illegal PstI site found at 3749
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2161
    Illegal BglII site found at 1454
    Illegal BglII site found at 1668
    Illegal BglII site found at 2578
    Illegal BamHI site found at 199
    Illegal BamHI site found at 2714
    Illegal BamHI site found at 3419
    Illegal XhoI site found at 2765
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2161
    Illegal PstI site found at 2920
    Illegal PstI site found at 3184
    Illegal PstI site found at 3283
    Illegal PstI site found at 3749
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2161
    Illegal PstI site found at 2920
    Illegal PstI site found at 3184
    Illegal PstI site found at 3283
    Illegal PstI site found at 3749
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 2223
    Illegal SapI.rc site found at 2367


Design Notes

The protein had to still be long enough to retain function. The sequence was used from US Patent 7892824.


Source

This sequence is shortened from the natural dystrophin sequence found in the body.

References