Part:BBa_M36953:Design

Insect antifreeze protein (RiAFP) actuator

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal SpeI site found at 220
  Illegal PstI site found at 517
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 602
  Illegal SpeI site found at 220
  Illegal PstI site found at 517
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal SpeI site found at 220
  Illegal PstI site found at 517
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal SpeI site found at 220
  Illegal PstI site found at 517
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

Codon optimization for human cell lines

Source

RiAFP: Rhagium Inquisitor

T2A: Thoseaasigna virus

References

Hakim A, Nguyen JB, Basu K, Zhu DF, Thakral D, Davies PL, Isaacs FJ, Modis Y, Meng W. Crystal structure of an insect antifreeze protein and its implications for ice binding. J Biol Chem. 2013 Apr 26;288(17):12295-304. doi: 10.1074/jbc.M113.450973. Epub 2013 Mar 12. PubMed PMID: 23486477; PubMed Central PMCID: PMC3636913.

Kim JH, Lee SR, Li LH, Park HJ, Park JH, Lee KY, Kim MK, Shin BA, Choi SY. High cleavage efficiency of a 2A peptide derived from porcine teschovirus-1 in human cell lines, zebrafish and mice. PLoS One. 2011;6(4):e18556. doi: 10.1371/journal.pone.0018556. Epub 2011 Apr 29. PubMed PMID: 21602908; PubMed Central PMCID: PMC3084703

FASTA

RiAFP Chain A:

>gi|460417438|pdb|4DT5|A Chain A, Crystal Structure Of Rhagium Inquisitor Antifreeze Protein GYSCRAVGVDGRAVTDIQGTCHAKATGAGAMASGTSEPGSTSTATATGRGATARSTSTGRGTATTTATGT ASATSNAIGQGTATTTATGSAGGRATGSATTSSSASQPTQTQTITGPGFQTAKSFARNTATTTVTASHHH HHH

RiAFP Chain B:

>gi|460417439|pdb|4DT5|B Chain B, Crystal Structure Of Rhagium Inquisitor Antifreeze Protein GYSCRAVGVDGRAVTDIQGTCHAKATGAGAMASGTSEPGSTSTATATGRGATARSTSTGRGTATTTATGT ASATSNAIGQGTATTTATGSAGGRATGSATTSSSASQPTQTQTITGPGFQTAKSFARNTATTTVTASHHH HHH