Part:BBa_M36939:Experience
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Applications of BBa_M36939
Stanford Location:
Box Name: BIOE44 S14
Glycerol Freezer Stocks:
Barcode#: 133010734, Plasmid Name: CME LCPUFA, Resistance Type: amp, DNA 2.0 Gene #:193424, Organism: E. coli, Device Type: LCPUFA Actuator.
Barcode#: 133009653, Plasmid Name: CME LCPUFA, Resistance Type: amp, DNA 2.0 Gene #:193424, Organism: E. coli, Device Type: LCPUFA Actuator.
Barcode#: 133026955, Plasmid Name: CME LCPUFA, Resistance Type: amp, DNA 2.0 Gene #:193424, Organism: E. coli, Device Type: LCPUFA Actuator.
Barcode#: 133024660, Plasmid Name: CME LCPUFA, Resistance Type: amp, DNA 2.0 Gene #:193424, Organism: E. coli, Device Type: LCPUFA Actuator.
Barcode#: 133024658, Plasmid Name: CME LCPUFA, Resistance Type: amp, DNA 2.0 Gene #:193424, Organism: E. coli, Device Type: LCPUFA Actuator.
Barcode#: 133024682, Plasmid Name: CME LCPUFA, Resistance Type: amp, DNA 2.0 Gene #:193424, Organism: E. coli, Device Type: LCPUFA Actuator.
Barcode#: 133024673, Plasmid Name: CME LCPUFA, Resistance Type: amp, DNA 2.0 Gene #:193424, Organism: E. coli, Device Type: LCPUFA Actuator.
Barcode#: 133024994, Plasmid Name: CME LCPUFA, Resistance Type: amp, DNA 2.0 Gene #:193424, Organism: E. coli, Device Type: LCPUFA Actuator.
Performance Data:
Protein Presence Test: GFP Fluorescence Reading
Note: GFP Fluorescence Readings were not taken for this composite part but for its sister part BBa_M36938 (contains Comet GFP). We suspect that similar results would be obtained for this construct (excluding Comet GFP). This data is included simply to provide a full analysis of the mechanism.
Conclusion: The target protein (enoyl-[acyl-carrier-protein] reductase) is synthesized within the E. coli cells. This result is statistically significant.
Protein Functionality Test: pH Reading
Functional (enoyl-[acyl-carrier-protein] reductase) is expected to partake in a naturally occurring reaction within the E. coli cell. This reaction consumes hydrogen ions. Thus a functional protein is expected to increase the overall pH of the cell.
Conclusion: Production of the target protein (enoyl-[acyl-carrier-protein] reductase) causes an increase in pH, as is to be expected. Therefore we conclude that the target protein is functional. This is statistically significant.
User Reviews
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