Part:BBa_M36921:Experience
Stanford Location
Plasmid Name: TL- Zinc Sensor.gdx
DNA2.0 Gene #: 193536
Organism: E. coli
Device Type: Sensor
Barcode #'s of Glycerol Stocks: 0133010718
Box Label: BioE44 S14 Box 1
Applications of BBa_M36921
This part has not been used in any other composite parts.
Performance Data of BBa_M36921
Our first trial had very promising results, showing an increase in fluorescence with increasing Zn2+ concentrations for our engineered plasmid. Our negative control plasmid (pJ1201-03c) also gave a low constant baseline of fluorescence. Unfortunately, in our second trial, we found that our negative control showed a similar "increasing" trend as the pZn. To test the statistical significance of our data from the second trial, we used a student t-Test. Our t-Test was a two-tailed test of our pZn and pJ1201 data populations assuming unequal variances. This test gave us p-value outputs that we compared with the threshold significance value of five percent. Two p-values were calculated (one for the sixteen hour reading & one for the twenty-four hour reading) for Zn2+ concentrations of 1000, 500, 1.95, and 0 mg/L and these values were used to try to disprove the null hypothesis that the means of the data populations are equal. Because half of our p-values were under .05 (0.026478414, 0.012607462, 0.035300697, 0.012522604) and half were over .05 (0.180998572, 0.291867597, 0.094258161, 0.335410155), the null hypothesis can not be invalidated, which shows that the data between our control plasmid and our engineered plasmid is statistically insignificant. However, this trial does not necessarily discredit our hypothesis and the functionality of our sensor. More trials need to be conducted with controlled variables and a greater number of data points are needed per Zn2+ concentration for more accurate p-values.
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