Coding

Part:BBa_M36825:Design

Designed by: Christine Chang, Sonia Targ   Group: Stanford BIOE44 - S11   (2014-10-23)


Phenylalanine Hydroxylase


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1141
    Illegal PstI site found at 700
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1141
    Illegal PstI site found at 700
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1141
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1141
    Illegal PstI site found at 700
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1141
    Illegal PstI site found at 700
    Illegal NgoMIV site found at 264
    Illegal NgoMIV site found at 1033
    Illegal AgeI site found at 712
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Design considerations included not having BsaI restriction sites, palindromic sequences, and whether the toxicity of the produced protein to E. coli (it is not).


Source

Phenylalanine hydroxylase comes from the the PAH gene, and the CDS for this specific part came from the human PAH gene.

References