Coding

Part:BBa_M36803:Design

Designed by: Richard Li   Group: Stanford BIOE44 - S11   (2014-04-30)


AlcR Gene


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 810
    Illegal XbaI site found at 880
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1692
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 810
    Illegal XbaI site found at 880
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 810
    Illegal XbaI site found at 880
    Illegal AgeI site found at 1561
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1345


Design Notes

Minimize >10 bp repeats to only 3.


Source

The AlcR sequence was generated from Talaromyces stipitatus and codon-optimized for E. coli.

References